Isolation and identification of lignans from pine needles of Pinus koraiensis Sieb. et Zucc.

Objective To study the lignans of pine needles of Pinus koraiensis Sieb.et Zucc.Methods Compounds were isolated by chromatography on silica gel,ODS,Sephadex LH-20 columns,and their structures were elucidated by their physicochemical properties and spectral analysis.Results Eight compounds were obtained and identified as(+)-isolariciresinol-9-O-β-D-xylopyranoside(1),(+)-isolariciresinol-9-O-β-D-glucopyranoside(2),7S,8R-threo-3′,4,9′-trihydroxy-3-methoxy-4′,7-epoxy-neolignan-9-O-α-L-rhamnopyranoside(3),7S,8R-threo-3′,9,9′-trihydroxy-3-methoxy-4′,7-epoxy-neolignan-4-O-α-L-rhamnopyranoside(4),7R,8S-erythro-4,7,9-trihydroxy-3,3′-dimethoxy-8-O-4′-neolignan-9′-O-α-L-rhamnopyranoside(5),7S,8S-threo-4,7,9-trihydroxy-3,3′-dimethoxy-8-O-4′-neolignan-9′-O-α-L-rhamnopyranoside(6),7S,8S-threo-3′,4,7,9-tetrahydroxy-3-methoxy-8-O-4′-neolignan-9′-O-α-L-rhamnopyranoside(7),7R,8S-erythro-3′,4,9,9′-tetrahydroxy-3-methoxy-8-O-4′-neolignan-7-O-β-D-glucopyranoside(8).Conclusions Compounds 18 are obtained from Pinus koraiensis Sieb.et Zucc.for the first time.