光合作用
淀粉
叶绿体
新陈代谢
碳水化合物代谢
碳水化合物
同化(音韵学)
呼吸
生物化学
化学
生物
植物
基因
语言学
哲学
作者
Carmen Hostettler,Katharina Kölling,Diana Santelia,Sebastian Streb,Oliver Kötting,Samuel C. Zeeman
出处
期刊:Methods in molecular biology
日期:2011-01-01
卷期号:: 387-410
被引量:51
标识
DOI:10.1007/978-1-61779-237-3_21
摘要
Starch is a primary product of photosynthesis in the chloroplasts of many higher plants. It plays an important role in the day-to-day carbohydrate metabolism of the leaf, and its biosynthesis and degradation represent major fluxes in plant metabolism. Starch serves as a transient reserve of carbohydrate which is used to support respiration, metabolism, and growth at night when there is no production of energy and reducing power through photosynthesis, and no net assimilation of carbon. The chapter includes techniques to measure starch amount and its rate of biosynthesis, to determine its structure and composition, and to monitor its turnover. These methods can be used to investigate transitory starch metabolism in Arabidopsis, where they can be applied in combination with genetics and systems-level approaches to yield new insight into the control of carbon allocation generally, and starch metabolism specifically. The methods can also be applied to the leaves of other plants with minimal modifications.
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