Modulating the Activity of Enzyme in Metal–Organic Frameworks Using the Photothermal Effect of Ti3C2 Nanosheets

Enzymes are versatile catalysts with high potential in various applications, and much attention has been paid to the stability improvement of native enzymes and activity modulation. Encapsulation in metal-organic frameworks (MOFs) as an efficient strategy for protecting fragile native enzymes while modulating the activity of enzymes remotely, which is practically demanded, has rarely been explored in MOF-encapsulated enzymes. Herein, Ti3C2 nanosheets exhibiting photothermal effect and biocompatibility were encapsulated in Cyt c-embedded ZIF-8 to tailor the enzymatic activity remotely by near-infrared (NIR) irradiation for the first time. By exposure to NIR light, the temperature of an aqueous solution containing Ti3C2/Cyt c@ZIF-8 increases obviously (up to 15 °C), while that of Cyt c@ZIF-8 shows no change. The enzymatic activity in the composites with a certain amount of nanosheets increases, which is attributed to the created defect and transformed microenvironment caused by the introduction of nanosheets. Importantly, the enzymatic activity in ZIF-8 can be further enhanced up to 150% under NIR light irradiation, and this enhancement can be modulated flexibly by varying laser power density. Our investigations indicate that Ti3C2 nanosheets are promising candidates for modulating the activity of encapsulated enzymes remotely.