Pseudomonas germanica sp. nov., isolated from Iris germanica rhizomes

Through bacterial plant–endophyte extraction from rhizomes of Iris germanica plant, a Gram-stain-negative, aerobic, catalase- and oxidase-positive gammaproteobacterial strain, referred to as FIT28 T , was isolated. FIT28 T shows vigorous growth on nutrient rich media within the temperature range of 4–35 °C, with optimal growth at 28 °C, a wide pH tolerance from pH 5 to 11, and salt tolerance up to 6 % (w/v) NaCl. Colonies are white-yellow and quickly become mucoid. The results of analysis of the 16S rRNA gene sequence placed the strain within the genus Pseudomonas , and multilocus sequence analysis (MLSA) using 16S rRNA, rpoB , gyrB and rpoD concatenated sequences revealed that the closest relatives of FIT28 T are Pseudomonas zeae OE48.2 T , ' Pseudomonas crudilactis ' UCMA 17988, Pseudomonas tensinigenes ZA5.3 T , Pseudomonas helmanticensis OHA11 T , Pseudomonas baetica a390 T , Pseudomonas iridis P42 T , Pseudomonas atagonensis PS14 T and Pseudomonas koreensis Ps 9-14 T , within the Pseudomonas koreensis subgroup of the Pseudomonas fluorescens lineage. The genome size of FIT28 T is about 6.7 Mb with 59.09 mol% DNA G+C content. Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values calculated from the genomic sequences of FIT28 T , and the closely related P. zeae OE48.2 T are 95.23 and 63.4 %, respectively. Biochemical, metabolic and chemotaxonomic studies further support our proposal that Pseudomonas germanica sp. nov., should be considered a novel species of the genus Pseudomonas . Hence, the type strain FIT28 T (=LMG 32353 T =DSM 112698 T ) has been deposited in public cell-type culture centres.