Distinctive Microbial Processes and Controlling Factors Related to Indirect N2O Emission from Agricultural and Urban Rivers in Taihu Watershed

Inland rivers are hotspots of anthropogenic indirect nitrous oxide (N₂O) emissions, but the underlying microbial processes remain poorly understood. This study measured N₂O fluxes from agricultural and urban rivers in Taihu watershed and investigated the microbial processes driving N₂O production and consumption. The N₂O fluxes were significantly higher in agricultural rivers (140.1 ± 89.1 μmol m^-2 d^-1) than in urban rivers (25.1 ± 27.0 μmol m^-2 d^-1) (p < 0.001). All wind-based models significantly underestimated N₂O flux in urban rivers (p < 0.05) when using the Intergovernmental Panel on Climate Change method because they underestimated the N₂O emission factor (EF_5r). Wind speed and nitrate were the key factors affecting N₂O fluxes in agricultural and urban rivers, respectively. NirK-type denitrifiers produced N₂O in urban river water, while nirS-type denitrifiers consumed N₂O in the sediments of all rivers. Co-occurrence network analysis indicated organics from Microcystis served as electron donors for denitrifiers (dominated by Flavobacterium) in water, while direct interspecies electron transfer between Thiobacillus and methanogens and between Dechloromonas and sulfate-reducing bacteria enhanced N₂O reduction in sediments. This study advances our knowledge on the distinctive microbial processes that determine N₂O emissions in inland rivers and illustrates the need to revise EF_5r for N₂O estimation in urban rivers.