已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

Multiomics Analysis Reveals Distinct Immunogenomic Features of Lung Cancer with Ground-Glass Opacity

肺癌 免疫系统 磨玻璃样改变 医学 病理 转录组 癌症研究
作者
Kezhong Chen,Jing Bai,Alexandre Reuben,Heng Zhao,Guannan Kang,Chunliu Zhang,Qingyi Qi,Yaping Xu,Shawna Hubert,Lianpeng Chang,Yanfang Guan,Lin Feng,Kai Zhang,Kezhong Chen,Xin Yi,Xuefeng Xia,Shujun Cheng,Fan Yang,Jianjun Zhang,Jun Wang
出处
期刊:American Journal of Respiratory and Critical Care Medicine [American Thoracic Society]
卷期号:204 (10): 1180-1192 被引量:5
标识
DOI:10.1164/rccm.202101-0119oc
摘要

Rationale: Ground-glass opacity (GGO)-associated lung cancers are common and radiologically distinct clinical entities known to have an indolent clinical course and superior survival, implying a unique underlying biology. However, the molecular and immune characteristics of GGO-associated lung nodules have not been systemically studied. Objectives: To provide mechanistic insights for the treatment of these radiologically distinct clinical entities. Methods: We initiated a prospective cohort study to collect and characterize pulmonary nodules with GGO components (nonsolid and part-solid nodules) or without GGO components, as precisely quantified by using three-dimensional image reconstruction to delineate the molecular and immune features associated with GGO. Multiomics assessment conducted by using targeted gene panel sequencing, RNA sequencing, TCR (T-cell receptor) sequencing, and circulating tumor DNA detection was performed. Measurements and Main Results: GGO-associated lung cancers exhibited a lower tumor mutation burden than solid nodules. Transcriptomic analysis revealed a less active immune environment in GGO components and immune pathways, decreased expression of immune activation markers, and less infiltration of most immune-cell subsets, which was confirmed by using multiplex immunofluorescence. Furthermore, T-cell repertoire sequencing revealed lower T-cell expansion in GGO-associated lung cancers. HLA loss of heterozygosity was significantly less common in lung adenocarcinomas with GGO components than in those without. Circulating tumor DNA analysis suggested that the release of tumor DNA to the peripheral blood was correlated with the tumor size of non-GGO components. Conclusions: Compared with lung cancers presenting with solid lung nodules, GGO-associated lung cancers are characterized by a less active metabolism and a less active immune microenvironment, which may be the mechanisms underlying their indolent clinical course. Clinical trial registered with www.clinicaltrials.gov (NCT03320044).
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
领导范儿应助kelvin采纳,获得10
1秒前
12369完成签到,获得积分10
2秒前
李健的粉丝团团长应助guo采纳,获得10
3秒前
3秒前
li完成签到,获得积分10
3秒前
陆陆大人发布了新的文献求助10
3秒前
香蕉惜霜发布了新的文献求助10
4秒前
搜集达人应助任鲂采纳,获得10
5秒前
张任的die完成签到 ,获得积分10
5秒前
Hello应助小何HUHU采纳,获得10
6秒前
失眠的霸完成签到,获得积分10
7秒前
乐乐应助开朗板栗采纳,获得10
7秒前
自由的青亦应助烂漫烧鹅采纳,获得20
7秒前
li发布了新的文献求助10
9秒前
Werido完成签到 ,获得积分10
9秒前
明理依云发布了新的文献求助20
10秒前
10秒前
11秒前
12秒前
13秒前
在水一方应助简单的紫易采纳,获得10
14秒前
16秒前
鲤鱼完成签到,获得积分10
17秒前
科目三应助黑魔仙小玥采纳,获得10
18秒前
李健应助饱满跳跳糖采纳,获得10
18秒前
任鲂发布了新的文献求助10
18秒前
19秒前
美丽的金鑫完成签到 ,获得积分10
19秒前
丘比特应助FFFFF采纳,获得10
20秒前
高挑的白旋风完成签到,获得积分10
20秒前
拒绝头秃完成签到 ,获得积分20
22秒前
wjy959完成签到,获得积分20
24秒前
24秒前
24秒前
25秒前
25秒前
cyw发布了新的文献求助10
27秒前
zouyingjie完成签到,获得积分10
27秒前
陆陆大人发布了新的文献求助10
28秒前
xiayu发布了新的文献求助20
29秒前
高分求助中
Sustainability in Tides Chemistry 2000
Bayesian Models of Cognition:Reverse Engineering the Mind 888
Essentials of thematic analysis 700
A Dissection Guide & Atlas to the Rabbit 600
Very-high-order BVD Schemes Using β-variable THINC Method 568
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 500
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3125687
求助须知:如何正确求助?哪些是违规求助? 2775981
关于积分的说明 7728751
捐赠科研通 2431479
什么是DOI,文献DOI怎么找? 1292070
科研通“疑难数据库(出版商)”最低求助积分说明 622334
版权声明 600376