化学
微流控
单细胞分析
微通道
电感耦合等离子体质谱法
吞吐量
纳米颗粒
纳米技术
质谱法
分析化学(期刊)
色谱法
细胞
材料科学
电信
生物化学
计算机科学
无线
作者
Zhenna Chen,Beibei Chen,Man He,Bin Hu
标识
DOI:10.1021/acs.analchem.1c04216
摘要
High-throughput single-cell analysis is critical to elucidate the cell heterogeneity. Recently, droplet microchips using oil/gas phases to generate single-cell encapsulated droplets have been combined with inductively coupled plasma-mass spectrometry (ICP-MS) for determination of trace elements in single cells with a throughput of dozens of cells per min. To improve the sample throughput and avoid the oil phase introduced into ICP-MS, herein, a negative magnetophoresis focusing microchip was established and online-coupled to ICP-MS for single-cell analysis. MCF-7 cells in the paramagnetic salt solution were introduced into the designed focusing microchannel, in which they were focused into a single stream under both the magnetic repulsion force and inertial lift force, and then were introduced into ICP-MS for online single-cell analysis. The important parameters including the chip design, the concentration of the paramagnetic salt solution, flow rate, cell density, and dwell time were optimized. Under the optimal conditions, a high sample throughput of 1390 cells min-1 was obtained. The established online analytical system was applied to study the uptake behaviors of MCF-7 cells for Zn2+ and ZnO nanoparticles (NPs) at a single-cell level. The single-cell analysis results indicate that MCF-7 cells displayed more remarkable heterogeneity when they were treated with ZnO NPs, and the uptake content of ZnO NPs by MCF-7 cells was less than that of Zn2+. Compared with other droplet microdevice-ICP-MS analysis systems, the developed system has the advantages of simple design and fabrication, no organic phase, a high throughput, and a low sample consumption (only 5 μL).
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