脂肪细胞
脂肪生成
基质血管部分
脂肪组织
白色脂肪组织
转录组
生物
细胞生物学
细胞
内分泌学
遗传学
基因表达
基因
作者
Katie L. Whytock,Yifei Sun,Adeline Divoux,Gongxin Yu,Steven R. Smith,Martin J. Walsh,Lauren M. Sparks
出处
期刊:iScience
[Elsevier]
日期:2022-08-01
卷期号:25 (8): 104772-104772
被引量:23
标识
DOI:10.1016/j.isci.2022.104772
摘要
White adipose tissue (WAT) is a complex mixture of adipocytes and non-adipogenic cells. Characterizing the cellular composition of WAT is critical for identifying where potential alterations occur that impact metabolism. Most single-cell (sc) RNA-Seq studies focused on the stromal vascular fraction (SVF) which does not contain adipocytes and have used technology that has a 3′ or 5′ bias. Using full-length sc/single-nuclei (sn) RNA-Seq technology, we interrogated the transcriptional composition of WAT using: snRNA-Seq of whole tissue, snRNA-Seq of isolated adipocytes, and scRNA-Seq of SVF. Whole WAT snRNA-Seq provided coverage of major cell types, identified three distinct adipocyte clusters, and was capable of tracking adipocyte differentiation with pseudotime. Compared to WAT, adipocyte snRNA-Seq was unable to match adipocyte heterogeneity. SVF scRNA-Seq provided greater resolution of non-adipogenic cells. These findings provide critical evidence for the utility of sc full-length transcriptomics in WAT and SVF in humans.
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