A smart microhydrogel membrane sensor realized by pipette tip

In this paper, we describe a simple and practical way to prepare hydrogel membranes in a conical channel (pipette tip). We used a pipette to create a gas pressure difference on both sides of the gel precursor, which drove the gel precursor to move in the pipette tip. During movement, the shape of the hydrogel precursor gradually becomes thinner as the radius of the tapered channel becomes larger. We use this principle to realize the highly controllable preparation of the hydrogel membrane structure (130 μm at its thinnest). Moreover, we fabricated a hydrogel membrane sensor in one step by implanting smart molecules in the hydrogel, which achieved rapid and sensitive detection of 0.5 μM-500 mM potassium ions. This method of preparing the hydrogel membrane sensor does not rely on professional membrane production equipment and complex molecular design processes, has high gel utilization and simple and controllable membrane thickness, and has a wide range of application value in the field of intelligent hydrogel-based analysis technology.