重组DNA
体外
神经毒素
融合蛋白
细胞
细胞培养
化学
体内
电池类型
细胞穿透肽
肉毒神经毒素
细胞生物学
生物化学
生物
毒素
基因
生物技术
遗传学
作者
Wei Xuan,Jiage Hu,Liling Jiang,Lü Li,Jia Liu
出处
期刊:STAR protocols
[Elsevier]
日期:2023-03-01
卷期号:4 (1): 102026-102026
被引量:1
标识
DOI:10.1016/j.xpro.2022.102026
摘要
We recently reported that fusion of cell-penetrating peptides (CPPs) to botulinum neurotoxin type A (BoNTA) proteins could improve the efficiency of cellular uptake. Here, we describe steps to produce and evaluate CPP-BoNTA fusion proteins. We present procedures for the expression and purification of recombinant CPP-BoNTA using insect-cell-based baculovirus expression vector system and in vitro characterization of purified proteins. We also detail the analysis of cellular uptake in cell culture and examination of the in vivo performance in mice. For complete details on the use and execution of this protocol, please refer to Wei et al. (2022).1.
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