SH-SY5Y型
氧化应激
活性氧
立体化学
化学
异核单量子相干光谱
MTT法
部分
抗氧化剂
绝对构型
化学结构
水解物
生物化学
细胞凋亡
二维核磁共振波谱
细胞培养
生物
有机化学
神经母细胞瘤
遗传学
水解
作者
Meichen Li,Yunqiang Zhang,Tingting Han,Lu Guan,Dongxue Fan,Qinke Wu,Jianyu Liu,Yongnan Xu,Yanhua Fan
出处
期刊:Phytochemistry
[Elsevier BV]
日期:2022-10-30
卷期号:205: 113487-113487
被引量:1
标识
DOI:10.1016/j.phytochem.2022.113487
摘要
Eleven undescribed derivatives of flavan, including flavan-3,4-diols vernicinosides A-H and profisetinidin-type-4-arylflavan-3-ols verniciflavanols A-C, together with eight known compounds were purified from the heartwood of Toxicodendron vernicifluum. The chemical structures of the undescribed compounds were characterized by spectroscopic data interpretation, including NMR (1H and 13C NMR HSQC and HMBC) and HRESIMS analysis. CD data analysis was conducted to assign the absolute configurations of the undescribed compounds and the active compound verniciflavanol A was also confirmed by ECD experiment. The absolute configuration of the sugar moiety was identified by GC analysis of chiral derivatives in the hydrolysate. MTT assay was applied to test these compounds against H2O2-induced oxidative stress in human neuroblastoma SH-SY5Y cells. Results found that verniciflavanol A demonstrated the best potential in protecting SH-SY5Y cells against H2O2-induced oxidative stress by inhibiting cell apoptosis and attenuate reactive oxygen species (ROS) level and mitochondrial dysfunction. And the underlying mechanism was confirmed to be associated with Nrf2-antioxidant response element signaling and IL-6 cell survival pathways.
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