膜
肽
生物物理学
化学
脂质双层
双层
线粒体内膜
线粒体
类胡萝卜素
细胞生物学
生物化学
生物
抗菌肽
作者
Xin Jiang,Chenguang Yang,Jie Qiu,Dongfei Ma,Cheng Xu,Shuxin Hu,Weijing Han,Bing Yuan,Ying Lü
出处
期刊:Nanoscale
[The Royal Society of Chemistry]
日期:2022-01-01
卷期号:14 (47): 17654-17660
被引量:4
摘要
LL-37, the only human host cathelicidin peptide, is proposed to be able to induce host cell apoptosis through mitochondrial membrane permeabilization (MMP). Detailed pathways of the LL-37-triggered MMP are however still disputed. It is generally believed that cationic peptides permeate a membrane mostly in conditions of micromolar peptide concentrations and negatively charged membranes, which are not usually satisfied in the mitochondrial circumstance. Herein, using a variety of single-molecule techniques, we show that nanomolar LL-37 specifically induces permeability of a phosphoethanolamine (PE)-rich biomimetic mitochondrial membrane in a protein-independent manner. The insertion dynamics of single LL-37 molecules exhibit different metastable states in bilayers composed of different lipids. Moreover, the PE lipids significantly facilitate adsorption and accumulation of LL-37 on the PE-rich bilayer, and produce deeper insertion of peptide oligomers, especially tetramers, into the bilayer. This work offers an alternative pathway of the LL-37-triggered MMP and apoptosis.
科研通智能强力驱动
Strongly Powered by AbleSci AI