Membrane Fusion Biophysical Analysis of Fusogenic Liposomes

等温滴定量热法 化学 POPC公司 脂质双层融合 脂质体 小泡 融合 Zeta电位 脂质双层 囊泡融合 生物物理学 化学工程 纳米技术 生物化学 材料科学 纳米颗粒 工程类 哲学 突触小泡 生物 语言学
作者
Rafaela R.M. Cavalcanti,Rafael B. Lira,Karin A. Riske
出处
期刊:Langmuir [American Chemical Society]
卷期号:38 (34): 10430-10441 被引量:22
标识
DOI:10.1021/acs.langmuir.2c01169
摘要

Liposomes represent important drug carrier vehicles in biological systems. A fusogenic liposomal system composed of equimolar mixtures of the cationic lipid DOTAP and the phospholipid DOPE showed high fusion and delivery efficiencies with cells and lipid vesicles. However, aspects of the thermodynamics involving the interaction of these fusogenic liposomes and biomimetic systems remain unclear. Here, we investigate the fusion of this system with large unilamellar vesicles (LUVs) composed of the zwitterionic lipid POPC and increasing fractions of the anionic lipid POPG and up to 30 mol % cholesterol. The focus here is to concomitantly follow changes in size, zeta-potential, and enthalpy binding upon membrane interaction and fusion. Isothermal titration calorimetry (ITC) data showed that membrane fusion in our system is an exothermic process in the absence of cholesterol, suggesting that electrostatic attraction is the driving force for fusion. An endothermic component appeared and eventually dominated the titration at 30 mol % cholesterol, which we propose is caused by membrane fluidification when cholesterol is diluted upon fusion. The inflection points of the ITC data occurred around 0.5–0.7 POPG/DOTAP for all systems, the same stoichiometry for which zeta-potential and dynamic light scattering measurements showed an increase in size coupled with charge neutralization of the system, which is consistent with the fact that fusion in our system is charge-mediated. Microscopy observations of the final mixtures revealed the presence of giant vesicles, which is a clear indication of fusion, coexisting with intermediate-sized objects that could be the result of both fusion and/or aggregation. The results show that the fusion efficiency of the DOTAP:DOPE fusogenic system is modulated by the charge and membrane packing of the acceptor membrane and explain why the system fuses very efficiently with cells.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
小井盖完成签到 ,获得积分10
1秒前
热情的元芹完成签到,获得积分10
1秒前
2秒前
陶醉的小海豚完成签到,获得积分10
3秒前
陆晓亦完成签到,获得积分10
3秒前
乐观的觅松完成签到,获得积分10
3秒前
2023204306324发布了新的文献求助10
4秒前
5秒前
端己完成签到,获得积分20
5秒前
6秒前
阿湫发布了新的文献求助10
6秒前
7秒前
7秒前
坤坤完成签到,获得积分10
7秒前
8秒前
STUSSY完成签到,获得积分10
8秒前
wuhuofeng发布了新的文献求助10
9秒前
10秒前
11秒前
11秒前
coco完成签到,获得积分10
12秒前
lshao完成签到 ,获得积分10
13秒前
13秒前
zhou发布了新的文献求助30
14秒前
跋扈完成签到,获得积分10
16秒前
温柔翰发布了新的文献求助10
16秒前
16秒前
Jj发布了新的文献求助10
17秒前
ficus_min发布了新的文献求助10
17秒前
木子发布了新的文献求助10
18秒前
Galato发布了新的文献求助10
18秒前
寒冷哈密瓜完成签到 ,获得积分0
18秒前
大模型应助shen采纳,获得10
19秒前
123566完成签到,获得积分10
19秒前
hohn完成签到,获得积分10
19秒前
科研通AI2S应助bsn采纳,获得10
21秒前
LL发布了新的文献求助10
21秒前
张西西完成签到 ,获得积分10
22秒前
研友_ZAxj7n完成签到,获得积分20
24秒前
海上钢琴家完成签到,获得积分10
24秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
Research on Disturbance Rejection Control Algorithm for Aerial Operation Robots 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038524
求助须知:如何正确求助?哪些是违规求助? 3576221
关于积分的说明 11374737
捐赠科研通 3305912
什么是DOI,文献DOI怎么找? 1819354
邀请新用户注册赠送积分活动 892688
科研通“疑难数据库(出版商)”最低求助积分说明 815048