Therapeutic Potential of the Combination of Pentoxifylline and Vitamin-E in Inflammatory Bowel Disease: Inhibition of Intestinal Fibrosis

医学 己酮可可碱 纤维化 炎症性肠病 体内 炎症 药理学 结肠炎 维生素E 免疫学 内科学 疾病 抗氧化剂 生物化学 化学 生物技术 生物
作者
Hyun Joo Lee
出处
期刊:Journal of Clinical Medicine [Multidisciplinary Digital Publishing Institute]
卷期号:11 (16): 4713-4713 被引量:13
标识
DOI:10.3390/jcm11164713
摘要

Although intestinal fibrosis is a consequence of recurrent inflammation in Inflammatory bowel disease (IBD), alleviating inflammation alone does not prevent the progression of fibrosis, suggesting that the development of direct anti-fibrotic agents is necessary. This study aimed to evaluate the anti-fibrotic properties of combination treatment with pentoxifylline (PTX) and vitamin E (Vit-E) on human primary intestinal myofibroblasts (HIMFs) and the therapeutic potential of the combination therapy in murine models of IBD.HIMFs were pretreated with PTX, Vit-E, or both, and incubated with TGF-β1. We performed Western blot, qPCR, collagen staining, and immunofluorescence to estimate the anti-fibrotic effects of PTX and Vit-E. The cytotoxicity of these was investigated through MTT assay. To induce murine models of IBD for in vivo study, C57BL/6 mice were treated with repeated cycles of dextran sulfate sodium (DSS), developing chronic colitis. We examined whether the combined PTX and Vit-E treatment would effectively ameliorate colonic fibrosis in vivo.We found that the co-treatment with PTX and Vit-E suppressed TGF-β1-induced expression of fibrogenic markers, with decreased expression of pERK, pSmad2, and pJNK, more than either treatment alone in HIMFs. Neither PTX nor Vit-E showed any significant cytotoxicity in given concentrations. Consistently with the in vitro results, the co-administration with PTX and Vit-E effectively attenuated colonic fibrosis with recovery from thickening and shortening of colon in murine models of IBD.These findings demonstrated that the combination of PTX and Vit-E exhibits significant anti-fibrotic effects in both HIMFs and in vivo IBD models, providing a promising therapy for IBD.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
健康的涔发布了新的文献求助10
1秒前
4秒前
秋暝寒衣完成签到,获得积分10
6秒前
完美世界应助bfs采纳,获得10
7秒前
橘子sungua完成签到,获得积分10
8秒前
111完成签到,获得积分10
8秒前
10秒前
12秒前
13秒前
可乐加冰完成签到,获得积分10
13秒前
David驳回了Ant应助
13秒前
14秒前
15秒前
量子星尘发布了新的文献求助10
16秒前
思源应助果粒多采纳,获得10
16秒前
17秒前
好滴捏发布了新的文献求助10
18秒前
bfs发布了新的文献求助10
19秒前
WN发布了新的文献求助10
20秒前
20秒前
慕青应助小白采纳,获得10
21秒前
AAACharlie发布了新的文献求助10
21秒前
热情的达发布了新的文献求助10
21秒前
orixero应助lucky李采纳,获得10
22秒前
22秒前
momo发布了新的文献求助10
23秒前
23秒前
24秒前
guo完成签到,获得积分10
25秒前
可期完成签到,获得积分10
26秒前
27秒前
wsj发布了新的文献求助10
27秒前
果粒多发布了新的文献求助10
28秒前
科目三应助ylq采纳,获得30
29秒前
liupc2019发布了新的文献求助20
30秒前
张雯思发布了新的文献求助10
33秒前
希望天下0贩的0应助momo采纳,获得10
33秒前
34秒前
35秒前
梦华完成签到 ,获得积分10
36秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989297
求助须知:如何正确求助?哪些是违规求助? 3531418
关于积分的说明 11253893
捐赠科研通 3270097
什么是DOI,文献DOI怎么找? 1804884
邀请新用户注册赠送积分活动 882087
科研通“疑难数据库(出版商)”最低求助积分说明 809158