Elucidation of interactions between myofibrillar proteins and κ-carrageenan as mediated by NaCl level: Perspectives on multiple spectroscopy and molecular docking

化学 范德瓦尔斯力 疏水效应 分子间力 氢键 溶解度 分子 荧光光谱法 对接(动物) Zeta电位 猝灭(荧光) 结晶学 立体化学 荧光 有机化学 化学工程 医学 物理 量子力学 工程类 纳米颗粒 护理部
作者
Chuanai Cao,Zicheng Zhu,Liang Xue,Baohua Kong,Zihan Xu,Pingru Shi,Yuangang Li,Yunlong Ji,Zixuan Ren,Qian Liu
出处
期刊:International Journal of Biological Macromolecules [Elsevier BV]
卷期号:248: 125903-125903 被引量:28
标识
DOI:10.1016/j.ijbiomac.2023.125903
摘要

The present study was aimed to investigate the intermolecular interaction between myofibrillar proteins (MP) and κ-carrageenan (KC) as mediated by KC concentration (0.1, 0.2, 0.3, and 0.4 %, w/w) and NaCl levels (0.3 and 0.6 M) based on the multiple spectroscopy and molecular docking. The results showed that the incorporation of KC increased the turbidity, zeta-potential, and surface hydrophobicity of MP-KC mixed sols with a dose-dependent manner, as well as significantly decreasing the protein solubility (P < 0.05), which indicated that the interaction between KC and MP promoted the expansion of protein structure and exposed more hydrophobic groups. Fluorescence spectra result revealed that the interaction between MP and KC was a static quenching in the fluorescence quenching process, which affected the aromatic amino acids residue microenvironment of MP. Moreover, the existence of KC decreased the α-helix contents of MP (P < 0.05), contributing to the transformation from random structure to organized configuration of MP. In addition, molecular forces, the molecular docking and thermodynamic parameters indicated that hydrophobic interactions, van der Waals force, and hydrogen bonding were considered as the main interaction forces between MP and KC. Furthermore, 0.6 M NaCl level rendered higher solubility and particle size, as well as lower turbidity and the surface hydrophobicity of MP-KC mixed sols than those with 0.3 M NaCl level (P < 0.05), which promoted the unfolding of MP molecule and subsequently increased the numbers of binding sites between MP and KC, facilitating the intermolecular interactions between MP and KC in mixed sols.
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