Elucidation of interactions between myofibrillar proteins and κ-carrageenan as mediated by NaCl level: Perspectives on multiple spectroscopy and molecular docking

化学 范德瓦尔斯力 疏水效应 分子间力 氢键 溶解度 分子 荧光光谱法 对接(动物) Zeta电位 猝灭(荧光) 结晶学 立体化学 荧光 有机化学 化学工程 医学 物理 量子力学 工程类 纳米颗粒 护理部
作者
Chuanai Cao,Zicheng Zhu,Liang Xue,Baohua Kong,Zihan Xu,Pingru Shi,Yuangang Li,Yunlong Ji,Zixuan Ren,Qian Liu
出处
期刊:International Journal of Biological Macromolecules [Elsevier BV]
卷期号:248: 125903-125903 被引量:28
标识
DOI:10.1016/j.ijbiomac.2023.125903
摘要

The present study was aimed to investigate the intermolecular interaction between myofibrillar proteins (MP) and κ-carrageenan (KC) as mediated by KC concentration (0.1, 0.2, 0.3, and 0.4 %, w/w) and NaCl levels (0.3 and 0.6 M) based on the multiple spectroscopy and molecular docking. The results showed that the incorporation of KC increased the turbidity, zeta-potential, and surface hydrophobicity of MP-KC mixed sols with a dose-dependent manner, as well as significantly decreasing the protein solubility (P < 0.05), which indicated that the interaction between KC and MP promoted the expansion of protein structure and exposed more hydrophobic groups. Fluorescence spectra result revealed that the interaction between MP and KC was a static quenching in the fluorescence quenching process, which affected the aromatic amino acids residue microenvironment of MP. Moreover, the existence of KC decreased the α-helix contents of MP (P < 0.05), contributing to the transformation from random structure to organized configuration of MP. In addition, molecular forces, the molecular docking and thermodynamic parameters indicated that hydrophobic interactions, van der Waals force, and hydrogen bonding were considered as the main interaction forces between MP and KC. Furthermore, 0.6 M NaCl level rendered higher solubility and particle size, as well as lower turbidity and the surface hydrophobicity of MP-KC mixed sols than those with 0.3 M NaCl level (P < 0.05), which promoted the unfolding of MP molecule and subsequently increased the numbers of binding sites between MP and KC, facilitating the intermolecular interactions between MP and KC in mixed sols.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
2秒前
2秒前
量子星尘发布了新的文献求助10
2秒前
3秒前
R2L23发布了新的文献求助10
5秒前
5秒前
诚心的扬发布了新的文献求助10
6秒前
7秒前
liupc2019完成签到,获得积分10
8秒前
顾矜应助momo采纳,获得10
9秒前
周em12_发布了新的文献求助10
9秒前
10秒前
11秒前
11秒前
FashionBoy应助ZZZ采纳,获得10
12秒前
14秒前
清爽傲云发布了新的文献求助10
16秒前
16秒前
18秒前
20秒前
嗨嗨害发布了新的文献求助10
20秒前
Eileen发布了新的文献求助10
21秒前
呵呵哒发布了新的文献求助10
24秒前
清爽傲云完成签到,获得积分10
26秒前
26秒前
27秒前
momo发布了新的文献求助10
31秒前
诚心的扬关注了科研通微信公众号
32秒前
34秒前
嗨嗨害完成签到,获得积分10
35秒前
吧啦吧啦完成签到,获得积分10
35秒前
36秒前
37秒前
zero完成签到,获得积分10
38秒前
小二郎应助momo采纳,获得10
39秒前
hh完成签到 ,获得积分10
39秒前
大大完成签到 ,获得积分10
39秒前
39秒前
量子星尘发布了新的文献求助10
41秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989297
求助须知:如何正确求助?哪些是违规求助? 3531418
关于积分的说明 11253893
捐赠科研通 3270097
什么是DOI,文献DOI怎么找? 1804884
邀请新用户注册赠送积分活动 882087
科研通“疑难数据库(出版商)”最低求助积分说明 809158