A versatile and high-throughput flow-cell system combined with fluorescence imaging for simultaneous single-molecule force measurement and visualization

生物分子 可视化 荧光 材料科学 跟踪(教育) 纳米技术 力谱学 分子 生物物理学 DNA 荧光寿命成像显微镜 生物系统 计算机科学 原子力显微镜 化学 光学 物理 人工智能 生物 有机化学 生物化学 教育学 心理学
作者
Zhenyu Zou,Jialun Liang,Qian Jia,Di Bai,Wei Xie,Wenqiang Wu,Chuang Tan,Jie Ma
出处
期刊:Nanoscale [Royal Society of Chemistry]
卷期号:15 (43): 17443-17454 被引量:1
标识
DOI:10.1039/d3nr03214k
摘要

A flow-cell offers many advantages for single-molecule studies. But, its merit as a quantitative single-molecule tool has long been underestimated. In this work, we developed a gas-pumped fully calibrated flow-cell system combined with fluorescence imaging for simultaneous single-molecule force measurement and visualization. Such a flow-cell system has considered the hydrodynamic drags on biomolecules and hence can apply and measure force up to more than 100 pN in sub-pN precision with an ultra-high force stability (force drift <0.01 pN in 10 minutes) and tuning accuracy (∼0.04 pN). Meanwhile, it also allows acquiring force signals and fluorescence images at the same time, parallelly tracking hundreds of protein motors in real time as well as monitoring the conformational changes of biomolecules under a well-controlled force, as demonstrated by a series of single-molecule experiments in this work, including the studies of DNA overstretching dynamics, transcription under force and DNA folding/unfolding dynamics. Interesting findings, such as the very tight association of single-stranded binding (SSB) proteins with ssDNA and the reversed transcription, have also been made. These results together lay down an essential foundation for a flow-cell to be used as a versatile, quantitative and high-throughput tool for single-molecule manipulation and visualization.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
古夕完成签到,获得积分10
1秒前
2秒前
3秒前
3秒前
5秒前
lm发布了新的文献求助10
5秒前
无所归兮应助曲艺采纳,获得10
6秒前
6秒前
yar应助alone采纳,获得30
7秒前
za==应助小赵采纳,获得10
7秒前
8秒前
豆芽发布了新的文献求助10
8秒前
oky发布了新的文献求助10
8秒前
wdy111应助迷路硬币采纳,获得20
10秒前
10秒前
11秒前
艺高人胆大鸡腿完成签到 ,获得积分10
14秒前
乐乐应助焦糖采纳,获得10
14秒前
科研通AI2S应助nalan采纳,获得10
15秒前
静_完成签到 ,获得积分10
15秒前
15秒前
雪白元蝶发布了新的文献求助10
16秒前
16秒前
16秒前
留白完成签到 ,获得积分10
17秒前
共享精神应助小圆采纳,获得10
17秒前
17秒前
慕青应助梵高的向日葵采纳,获得10
17秒前
SYLH应助科研通管家采纳,获得20
17秒前
czh应助科研通管家采纳,获得10
17秒前
17秒前
ding应助科研通管家采纳,获得10
17秒前
搜集达人应助科研通管家采纳,获得10
18秒前
打打应助科研通管家采纳,获得10
18秒前
18秒前
彭于彦祖应助科研通管家采纳,获得10
18秒前
彭于彦祖应助科研通管家采纳,获得30
18秒前
雯雯完成签到,获得积分10
18秒前
18秒前
18秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Economic Geography and Public Policy 900
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3988786
求助须知:如何正确求助?哪些是违规求助? 3531116
关于积分的说明 11252493
捐赠科研通 3269766
什么是DOI,文献DOI怎么找? 1804771
邀请新用户注册赠送积分活动 881870
科研通“疑难数据库(出版商)”最低求助积分说明 809021