Time-resolved spectroscopic and computational study of the initial events in doxazosin photochemistry

Doxazosin is a quinazoline derivative widely used in medicine as a drug. In this study, a combined experimental and computational approach based on the time-dependent density functional theory was used to elucidate the primary events following the photoexcitation of DOX upon interaction with light. The photophysical properties and photochemical reactivity of DOX were investigated by steady-state and time-resolved absorption and fluorescence spectroscopy. DOX in H2O in S0 is present in two prototropic forms, with the protonated form dominating (∼91 %, pKa = 6.75). The computations indicated that the most basic quinazoline nitrogen is at the position 1. Upon excitation, DOX deprotonates in the singlet excited state (pKa* = 1.31), and the decay times from the singlet excited state of 5 ns and 13 ns are attributed to the non-protonated and protonated forms of DOX, respectively. The quantum yield of fluorescence in H2O is 0.51 and 0.64 in basic media. The quantum yield of intersystem crossing along with triplet–triplet molar absorption coefficient at 520 nm and the lifetime of the triplet excited state were obtained by LFP, ΦISC = 0.17, ε520 = 11600 ± 100 M−1 cm−1 and τ = 11 μs, respectively. Furthermore, LFP enabled detection of DOX radical formed by the photoinduced intramolecular electron transfer from the benzodioxane-carbamoyl to the protonated aminoquinazoline. Computations were used to back up the assignments of the detected transients and to construct an energy diagram with all plausible photophysical and photochemical pathways. These results elucidated the mechanisms of DOX photochemistry leading to DOX photodegradation which is relevant to environmental studies. They also provided insights into the potential use of such a quinazoline derivative in other applications such as push–pull chromophores or fluorescent probes.