Liquiritin inhibits H 2 O 2 ‐induced oxidative stress injury in H9c2 cells via the AMPK/SIRT1/NF‐κB signaling pathway

Journal of Food BiochemistryEarly View e14351 ORIGINAL ARTICLE Liquiritin inhibits H2O2-induced oxidative stress injury in H9c2 cells via the AMPK/SIRT1/NF-κB signaling pathway Tong-Juan Tang, Tong-Juan Tang orcid.org/0000-0001-5570-9234 Graduate School of Anhui University of Chinese Medicine, Hefei, ChinaSearch for more papers by this authorXiang Wang, Xiang Wang Contribution: MethodologySearch for more papers by this authorLiang Wang, Liang Wang Department of Integrated Traditional Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei, China Research Institute of Integrated Traditional Chinese and Western Medicine, Anhui Academy of Chinese Medicine, Hefei, China Anhui Province Key Laboratory of Chinese Medicinal Formula, Anhui Academy of Chinese Medicine, Hefei, China Contribution: MethodologySearch for more papers by this authorMing Chen, Ming Chen Department of Integrated Traditional Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei, China Research Institute of Integrated Traditional Chinese and Western Medicine, Anhui Academy of Chinese Medicine, Hefei, China Anhui Province Key Laboratory of Chinese Medicinal Formula, Anhui Academy of Chinese Medicine, Hefei, China Contribution: MethodologySearch for more papers by this authorJing Cheng, Jing Cheng School of Nursing, Anhui University of Chinese Medicine, Hefei, China Contribution: MethodologySearch for more papers by this authorMeng-Yu Zuo, Meng-Yu Zuo Graduate School of Anhui University of Chinese Medicine, Hefei, China Contribution: Data curationSearch for more papers by this authorJin-Fan Gu, Jin-Fan Gu Graduate School of Anhui University of Chinese Medicine, Hefei, China Contribution: Data curationSearch for more papers by this authorRui Ding, Rui Ding Graduate School of Anhui University of Chinese Medicine, Hefei, China Contribution: Data curationSearch for more papers by this authorPeng Zhou, Corresponding Author Peng Zhou zhoupeng@ahtcm.edu.cn Department of Integrated Traditional Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei, China Research Institute of Integrated Traditional Chinese and Western Medicine, Anhui Academy of Chinese Medicine, Hefei, China Anhui Province Key Laboratory of Chinese Medicinal Formula, Anhui Academy of Chinese Medicine, Hefei, China Correspondence Peng Zhou and Jin-ling Huang, Department of Integrated Traditional Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei, 230012, China. Email: zhoupeng@ahtcm.edu.cn (P. Z.); jinling6181@126.com (J. H.) Contribution: Project administration, Writing - review & editingSearch for more papers by this authorJin-Ling Huang, Corresponding Author Jin-Ling Huang jinling6181@126.com Department of Integrated Traditional Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei, China Research Institute of Integrated Traditional Chinese and Western Medicine, Anhui Academy of Chinese Medicine, Hefei, China Anhui Province Key Laboratory of Chinese Medicinal Formula, Anhui Academy of Chinese Medicine, Hefei, China Correspondence Peng Zhou and Jin-ling Huang, Department of Integrated Traditional Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei, 230012, China. Email: zhoupeng@ahtcm.edu.cn (P. Z.); jinling6181@126.com (J. H.) Contribution: Conceptualization, Writing - review & editingSearch for more papers by this author Tong-Juan Tang, Tong-Juan Tang orcid.org/0000-0001-5570-9234 Graduate School of Anhui University of Chinese Medicine, Hefei, ChinaSearch for more papers by this authorXiang Wang, Xiang Wang Contribution: MethodologySearch for more papers by this authorLiang Wang, Liang Wang Department of Integrated Traditional Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei, China Research Institute of Integrated Traditional Chinese and Western Medicine, Anhui Academy of Chinese Medicine, Hefei, China Anhui Province Key Laboratory of Chinese Medicinal Formula, Anhui Academy of Chinese Medicine, Hefei, China Contribution: MethodologySearch for more papers by this authorMing Chen, Ming Chen Department of Integrated Traditional Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei, China Research Institute of Integrated Traditional Chinese and Western Medicine, Anhui Academy of Chinese Medicine, Hefei, China Anhui Province Key Laboratory of Chinese Medicinal Formula, Anhui Academy of Chinese Medicine, Hefei, China Contribution: MethodologySearch for more papers by this authorJing Cheng, Jing Cheng School of Nursing, Anhui University of Chinese Medicine, Hefei, China Contribution: MethodologySearch for more papers by this authorMeng-Yu Zuo, Meng-Yu Zuo Graduate School of Anhui University of Chinese Medicine, Hefei, China Contribution: Data curationSearch for more papers by this authorJin-Fan Gu, Jin-Fan Gu Graduate School of Anhui University of Chinese Medicine, Hefei, China Contribution: Data curationSearch for more papers by this authorRui Ding, Rui Ding Graduate School of Anhui University of Chinese Medicine, Hefei, China Contribution: Data curationSearch for more papers by this authorPeng Zhou, Corresponding Author Peng Zhou zhoupeng@ahtcm.edu.cn Department of Integrated Traditional Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei, China Research Institute of Integrated Traditional Chinese and Western Medicine, Anhui Academy of Chinese Medicine, Hefei, China Anhui Province Key Laboratory of Chinese Medicinal Formula, Anhui Academy of Chinese Medicine, Hefei, China Correspondence Peng Zhou and Jin-ling Huang, Department of Integrated Traditional Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei, 230012, China. Email: zhoupeng@ahtcm.edu.cn (P. Z.); jinling6181@126.com (J. H.) Contribution: Project administration, Writing - review & editingSearch for more papers by this authorJin-Ling Huang, Corresponding Author Jin-Ling Huang jinling6181@126.com Department of Integrated Traditional Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei, China Research Institute of Integrated Traditional Chinese and Western Medicine, Anhui Academy of Chinese Medicine, Hefei, China Anhui Province Key Laboratory of Chinese Medicinal Formula, Anhui Academy of Chinese Medicine, Hefei, China Correspondence Peng Zhou and Jin-ling Huang, Department of Integrated Traditional Chinese and Western Medicine, Anhui University of Chinese Medicine, Hefei, 230012, China. Email: zhoupeng@ahtcm.edu.cn (P. Z.); jinling6181@126.com (J. H.) Contribution: Conceptualization, Writing - review & editingSearch for more papers by this author First published: 05 August 2022 https://doi.org/10.1111/jfbc.14351Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Heart failure (HF) is a serious disease with high mortality. Oxidative stress plays a vital role in its occurrence and development. Licorice is commonly used to treat HF in traditional Chinese medicine. Liquiritin, the main ingredient of licorice, has antioxidant and anti-inflammatory properties, but the mechanism against oxidative stress in cardiomyocytes has not been reported. Establishment of oxidative damage model in H9c2 cells by hydrogen peroxide (H2O2). Liquiritin (5, 10, 20 μmol/L) could significantly prevent the loss of cell viability and decrease the apoptosis rate. It can reduce the levels of reactive oxygen species (ROS), malonedialdehyde (MDA), lactate dehydrogenase (LDH), tumor necrosis factor-alpha (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), and increase the activity of ATP, superoxidedismutase (SOD), glutathione peroxide (GSH-px), glutathione reductase (GR) and catalase (CAT) to alleviate oxidative stress and inflammation in a dose-dependent manner. Liquiritin was found to be related to AMP-Activated Protein Kinase (AMPK) pathway by molecular docking. Western blotting (WB) and quantitative reverse transcription PCR (RT-qPCR) confirmed that liquiritin could promote AMPKα phosphorylation and sirtuin 1 (SIRT1) protein expression, and inhibit phosphorylation of nuclear factor kappa B p65 (NF-κB p65). Compound C, EX 527, and PDTC can reverse the effects of liquiritin, indicating that its antioxidant effect is achieved by regulating AMPK/SIRT1/NF-κB signaling pathway. Practical applications Heart failure is one of the most common cardiovascular diseases, and its treatment remains a worldwide problem. Licorice is a food and dietary supplement that has been used widely in traditional Chinese medicine (TCM). Liquiritin is one of the main active components of licorice, which has antioxidant and anti-inflammatory pharmacological effects. This study revealed the mechanism of licorice against oxidative damage of H9c2 cardiomyocytes, and provided a scientific basis for liquiritin as an antioxidant in the treatment of heart failure. CONFLICT OF INTEREST The authors declare that they have no conflicts of interest. Open Research DATA AVAILABILITY STATEMENT The data that support the findings of this study are available on request from the corresponding author. Early ViewOnline Version of Record before inclusion in an issuee14351 RelatedInformation