TLR4型
多糖
化学
MAPK/ERK通路
NF-κB
THP1细胞系
甘露糖
单糖
葡萄糖醛酸
生物化学
信号转导
半乳糖
免疫系统
糖醛酸
脂多糖
药理学
生物
细胞培养
免疫学
遗传学
作者
Jianhua Wu,Juanfen Mo,Wei Xiang,Xiaowen Shi,Li Guo,Yang Li,Bao Yang,Zheng Li
标识
DOI:10.1016/j.biopha.2023.114471
摘要
Polysaccharide of Tetrastigma hemsleyanum (THP) exert antioxidant, antibacterial, lipid-lowering, and anti-inflammatory properties, especially some evidences have highlighted the efficiency of it as an anti-tumor agent. However, as a biological macromolecule with bidirectional immune regulation, the immunological enhancement effects of THP on macrophages and its underlying mechanisms are still largely unknown. In the present study, THP was prepared and characterized, and then the effect of THP on Raw264.7 cell activation was investigated. Structural characteristics of THP showed that the average molecular weight was 370.26 kDa, and the main monosaccharide composition was galactose, glucuronic acid, mannose, and glucose at a ratio of 31.56: 25.15: 19.44: 12.60, with high viscosity causing by relative high uronic acid. For immunomodulatory activity investigation, THP promoted the production of NO, IL-6 and TNF-α, as well as the expression of IL-1β, MCP-1, iNOS and COX-2, which were almost completely inhibited by TLR4 antagonist. Further study showed that THP could activate NF-κB and MAPK signaling pathways, and thus enhanced the phagocytic activity of Raw264.7 macrophages. In conclusion, the present study provided evidences that THP could be served as a new immunomodulator in both functional foods and the pharmaceutical field.
科研通智能强力驱动
Strongly Powered by AbleSci AI