生物
柑橘衰退病毒
清脆的
病毒学
病毒
鉴定(生物学)
计算生物学
植物病毒
遗传学
植物
基因
作者
Hae-Jun Kim,In-Sook Cho,Se-Ryung Choi,Rae‐Dong Jeong
出处
期刊:Phytopathology
[American Phytopathological Society]
日期:2023-12-11
卷期号:114 (6): 1421-1428
被引量:4
标识
DOI:10.1094/phyto-10-23-0354-r
摘要
Citrus tristeza virus (CTV) is a highly destructive viral pathogen posing a significant threat to citrus crops worldwide. Disease management and crop protection strategies necessitate the development of rapid and accurate detection methods. In this study, we employed Oxford Nanopore sequencing to detect CTV in Citrus unshiu samples. Subsequently, we developed a specific and sensitive detection assay combining CRISPR/Cas12a with reverse transcription-recombinase polymerase amplification. The CRISPR-Cas12a assay exhibited exceptional specificity for CTV, surpassing conventional RT-PCR by at least 10-fold in sensitivity. Remarkably, the developed assay detected CTV in field samples, with zero false negatives. This diagnostic approach is user-friendly, cost-effective, and offers tremendous potential for rapid onsite detection of CTV. Therefore, the CRISPR-Cas12a assay plays a significant role in managing and preserving citrus trees that are free from viruses in the industry.
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