硒
标准物质
化学
酵母
质谱法
砷
高效液相色谱法
环境化学
质量分数
色谱法
生物化学
检出限
有机化学
作者
Xiao Li,Ling Shi,Pengcheng Song,Cai Wei,Ximing Luo,Bo Zhao
出处
期刊:Molecules
[MDPI AG]
日期:2024-01-01
卷期号:29 (1): 235-235
被引量:1
标识
DOI:10.3390/molecules29010235
摘要
Selenium-enriched yeast possesses the unique ability of transforming chemical selenium, such as sodium selenite, into a biologically active form, which mitigates its toxic effects on the human body. The transformation product of this process, selenomethionine, can be safely and effectively absorbed and utilized by the human body; hence, it has been spiked into a selenium-enriched supplement. This study employs two distinct measurement strategies to determine the selenomethionine content in two candidate reference materials, a selenium-enriched yeast powder and supplement, using both organic and inorganic mass spectrometry. The concentrations of selenomethionine in the selenium-enriched yeast were determined using HPLC-ICP-MS and HPLC- ESI-MS/MS, with mass fractions measured at 718 mg SeMet kg−1 and 715 mg SeMet kg−1, respectively. Notably, both methods yielded consistent results for the selenium supplement, with a selenomethionine mass fraction of 59 mg SeMet kg−1. Ultimately, the certified values of these candidate reference materials were determined as 716 mg kg−1 and 59 mg SeMet kg−1 with expanded uncertainties of 36 mg SeMet kg−1 (k = 2) and 5 mg SeMet kg−1 (k = 2), respectively. The development of these candidate reference materials serves as a valuable reference for diverse methods aiming to determine the value of organic selenium speciation in complex food substrates.
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