Optical manipulation reveals macrophage mediated mesoscale brain mechanical homeostasisin vivo

间质细胞 体内 巨噬细胞 神经科学 材料科学 生物 病理 医学 体外 生物化学 生物技术
作者
Woong Young So,Bailey Johnson,Patricia B. Gordon,Kevin Bishop,Haihong Gong,Hannah A. Burr,Jack R. Staunton,Chenchen Handler,Raman Sood,Giuliano Scarcelli,Kandice Tanner
标识
DOI:10.1101/2023.12.27.573380
摘要

Abstract Tissues are active materials where epithelial turnover, immune surveillance, and remodeling of stromal cells such as macrophages all regulate form and function. Scattering modalities such as Brillouin microscopy (BM) can non-invasively access mechanical signatures at GHz. However, our traditional understanding of tissue material properties is derived mainly from modalities which probe mechanical properties at different frequencies. Thus, reconciling measurements amongst these modalities remains an active area. Here, we compare optical tweezer active microrheology (OT-AMR) and Brillouin microscopy (BM) to longitudinally map brain development in the larval zebrafish. We determine that each measurement is able to detect a mechanical signature linked to functional units of the brain. We demonstrate that the corrected BM-Longitudinal modulus using a density factor correlates well with OT-AMR storage modulus at lower frequencies. We also show that the brain tissue mechanical properties are dependent on both the neuronal architecture and the presence of macrophages. Moreover, the BM technique is able to delineate the contributions to mechanical properties of the macrophage from that due to colony stimulating factor 1 receptor (CSF1R) mediated stromal remodeling. Here, our data suggest that macrophage remodeling is instrumental in the maintenance of tissue mechanical homeostasis during development. Moreover, the strong agreement between the OT-AM and BM further demonstrates that scattering-based technique is sensitive to both large and minute structural modification in vivo .

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