l-Borneol and -Borneol promote transdifferentiation of astrocytes into neurons in rats by regulating Wnt/Notch pathway to exert neuroprotective effect during recovery from cerebral ischaemia

神经保护 医学 缺血 神经科学 内科学 生物
作者
Rong Ma,Danni Lu,Qian Xie,Jianmei Yuan,Mihong Ren,Yong Li,Jiajun Wang,Jinxiu Li,Zhuo Xu,Jian Wang
出处
期刊:Phytomedicine [Elsevier]
卷期号:109: 154583-154583 被引量:10
标识
DOI:10.1016/j.phymed.2022.154583
摘要

The Chinese medicines Borneolum and l-Borneolum have neuroprotective effects on acute cerebral ischaemia-reperfusion (IR) in rats. Research on their effects during recovery from cerebral IR is lacking. Cerebral ischaemia can activate astrocytes for conversion into neurons. Neurogenesis cannot be achieved without nutritional support from an improved brain microenvironment through the blood circulation.The purpose of this study was to determine whether Borneolum and l-Borneolum can promote transdifferentiation of astrocytes into neurons by regulating the Wnt/Notch pathway to exert neuroprotective effects during recovery from cerebral ischaemia.A suture crossing the external carotid artery to occlude the middle cerebral artery was used to prepare a model of cerebral IR (Longa et al., 1989). The Longa neurological function score, modified neurological severity score, tape removal test and grid misstep experiment were used to evaluate motor nerve function. Triphenyltetrazolium chloride was used to determine the extent of cerebral infarction. Left/right hemisphere contrast was used to measure brain atrophy. Astrocytes labelled with adeno-associated virus were used to track their fate after transdifferentiation. Laser speckle contrast imaging was used to observe the effects of l-Borneolum and Borneolum on cerebral blood flow. Immunofluorescence and western blotting were used to investigate their mechanisms.l-Borneolum and Borneolum significantly improved neurological function and limb movement in rats with cerebral ischaemia during recovery and increased cerebral blood flow. l-Borneolum improved forelimb motor coordination more effectively than Borneolum and promoted transdifferentiation of astrocytes to GABAergic neurons in the striatal region. The expression of Wnt3a and Notch-1 was downregulated. The expression of vascular endothelial growth factor was not significantly changed. Borneolum improved forelimb sensitivity and alleviated cerebral infarction and brain atrophy more effectively than l-Borneolum, which promoted transdifferentiation of astrocytes into neurons and nestin expression and neurogenesis in the striatal zone. The expression of glycogen synthase kinase-3β and β-catenin was upregulated. l-Borneolum and Borneolum had no significant neuroprotective effect on the cortex and hippocampus.l-Borneolum and Borneolum exerted neuroprotective effects on cerebral ischaemia during recovery by promoting neurogenesis and blood circulation in the striatal and subventricular zones. Their mechanisms may be related to the Wnt3a and Notch-1 pathways.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
周周有一只很丑的熊完成签到,获得积分10
1秒前
别时圆完成签到,获得积分10
1秒前
3秒前
3秒前
4秒前
5秒前
王永文完成签到,获得积分10
5秒前
7秒前
asd1576562308发布了新的文献求助30
8秒前
8秒前
8秒前
11111发布了新的文献求助10
8秒前
9秒前
10秒前
11秒前
牛牛发布了新的文献求助10
12秒前
大橘完成签到 ,获得积分10
12秒前
ppg123应助nushell采纳,获得10
12秒前
搞什么科研完成签到,获得积分20
14秒前
赘婿应助xwl9955采纳,获得10
14秒前
huhuhufox发布了新的文献求助10
15秒前
15秒前
jennie完成签到 ,获得积分10
17秒前
zhangpeng发布了新的文献求助10
17秒前
嗯哼应助tangtang采纳,获得20
18秒前
cdqiu完成签到,获得积分10
18秒前
19秒前
20秒前
邓焕然完成签到,获得积分10
22秒前
酷波er应助小张采纳,获得10
23秒前
Ryang完成签到,获得积分10
23秒前
24秒前
qmdx完成签到,获得积分10
24秒前
25秒前
果果发布了新的文献求助10
27秒前
无魇应助wuhan采纳,获得50
27秒前
李lailai完成签到,获得积分10
27秒前
27秒前
ppg123应助paobashan采纳,获得10
27秒前
28秒前
高分求助中
The late Devonian Standard Conodont Zonation 2000
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
Smart but Scattered: The Revolutionary Executive Skills Approach to Helping Kids Reach Their Potential (第二版) 1000
Very-high-order BVD Schemes Using β-variable THINC Method 830
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 800
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3248330
求助须知:如何正确求助?哪些是违规求助? 2891731
关于积分的说明 8268453
捐赠科研通 2559668
什么是DOI,文献DOI怎么找? 1388584
科研通“疑难数据库(出版商)”最低求助积分说明 650772
邀请新用户注册赠送积分活动 627744