运输机
镉
硝酸盐
化学
硝酸镉
细胞生物学
环境化学
生物化学
药理学
生物
基因
有机化学
作者
Hongfang Jia,Zitong Zhu,Jiawei Zhan,Yong Luo,Zhuoran Yin,Zhaojun Wang,Xiaoxiao Yan,Huifang Shao,Zhaopeng Song
标识
DOI:10.1016/j.jhazmat.2024.134719
摘要
Heavy metal cadmium (Cd) is widespread in contaminated soil and an important factor limiting plant growth. NO3- (nitrate) affects Cd uptake and thus changes Cd tolerance in plants; however, the underlying molecular regulatory mechanisms have not yet been elucidated. Here, we analyzed a novel gene, NtARF11 (auxin response factor), which regulates Cd tolerance in tobacco via the NO3- uptake pathway, through experiments with NtARF11-knockout and NtARF11-overexpression transgenic tobacco lines. NtARF11 was highly expressed under Cd stress in tobacco plants. Under Cd stress, overexpression of NtARF11 enhanced Cd tolerance in tobacco compared to that in wild-type tobacco, as shown by the low Cd concentration, high chlorophyll concentration, and low accumulation of reactive oxygen species in NtARF11-overexpressing tobacco. Moreover, low NO3- concentrations were observed in NtARF11-overexpressing tobacco plants. Further analyses revealed direct binding of NtARF11 to the promoter of the nitrate transporter NtNRT1.1, thereby negatively regulating its expression in tobacco. Notably, NtNRT1.1 knockout reduced NO3- uptake, which resulted in low Cd concentrations in tobacco. Altogether, these results demonstrate that the NtARF11–NtNRT1.1 module functions as a positive regulator of Cd tolerance by reducing the Cd uptake in tobacco, providing new insights for improving Cd tolerance of plants through genetic engineering. Genetic improvements could play a significant role in enhancing the Cd tolerance of plants. In this study, we focus on a novel Cd-responsive gene NtARF11 in tobacco, to investigate its tolerance to Cd and the underlying molecular mechanisms. Our findings demonstrates that overexpression of NtARF11 could reduce the Cd uptake and improve tobacco tolerance to Cd toxicity by inhibiting the expression of nitrate transporter NtNRT1.1. Importantly, the NtARF11-NtNRT1.1 module acts as a positive regulator in tobacco's Cd tolerance. This is the first study to provide direct evidence that NtARF11 could regulate Cd uptake thought nitrate absorption pathway.
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