亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Evaluation of the Vitek 2 system for antifungal susceptibility testing of Candida auris using a representative international panel of clinical isolates: overestimation of amphotericin B resistance and underestimation of fluconazole resistance

金念珠菌 氟康唑 两性霉素B 抗真菌 微生物学 真菌不全 生物 抗药性 白霉素类 真菌病 医学 免疫学 卡斯波芬金
作者
Maria Siopi,Ioannis Pachoulis,Sevasti Leventaki,Bram Spruijtenburg,Jacques F. Meis,Spyros Pournaras,Larisa Vasilieva,Athanassios Tsakris,Joseph Meletiadis
出处
期刊:Journal of Clinical Microbiology [American Society for Microbiology]
标识
DOI:10.1128/jcm.01528-23
摘要

ABSTRACT Although the Vitek 2 system is broadly used for antifungal susceptibility testing of Candida spp., its performance against Candida auris has been assessed using limited number of isolates recovered from restricted geographic areas. We therefore compared Vitek 2 system with the reference Clinical and Laboratory Standards Institute (CLSI) broth microdilution method using an international collection of 100 C . auris isolates belonging to different clades. The agreement ±1 twofold dilution between the two methods and the categorical agreement (CA) based on the Centers for Disease Control and Prevention’s (CDC’s) tentative resistance breakpoints and Vitek 2-specific wild-type upper limit values (WT-ULVs) were determined. The CLSI-Vitek 2 agreement was poor for 5-flucytosine (0%), fluconazole (16%), and amphotericin B (29%), and moderate for voriconazole (61%), micafungin (67%), and caspofungin (81%). Significant interpretation errors were recorded using the CDC breakpoints for amphotericin B (31% CA, 69% major errors; MaEs) and fluconazole (69% CA, 31% very major errors; VmEs), but not for echinocandins (99% CA, 1% MaEs for both micafungin and caspofungin) for which the Vitek 2 allowed correct categorization of echinocandin-resistant FKS1 mutant isolates. Discrepancies were reduced when the Vitek 2 WT-ULV of 16 mg/L for amphotericin B (98% CA, 2% MaEs) and of 4 mg/L for fluconazole (96% CA, 1% MaEs, 3% VmEs) were used. In conclusion, the Vitek 2 system performed well for echinocandin susceptibility testing of C .auris . Resistance to fluconazole was underestimated whereas resistance to amphotericin B was overestimated using the CDC breakpoints of ≥32 and ≥2 mg/L, respectively. Vitek 2 minimun inhibitory concentrations (MICs) >4 mg/L indicated resistance to fluconazole and Vitek 2 MICs ≤16 mg/L indicated non-resistance to amphotericin B.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
miracle完成签到 ,获得积分10
2秒前
sss发布了新的文献求助10
8秒前
华桦子完成签到 ,获得积分10
10秒前
凌灵翎完成签到,获得积分10
12秒前
lenon完成签到,获得积分10
13秒前
思源应助sss采纳,获得10
23秒前
28秒前
33秒前
欣喜的以丹完成签到,获得积分10
39秒前
一D发布了新的文献求助10
42秒前
muhum完成签到 ,获得积分10
48秒前
实验大牛完成签到,获得积分10
51秒前
852应助scholar丨崔采纳,获得10
1分钟前
1分钟前
一D完成签到,获得积分10
1分钟前
儒雅致远发布了新的文献求助10
1分钟前
YiPeng完成签到,获得积分10
1分钟前
1分钟前
核桃发布了新的文献求助10
1分钟前
桐桐应助儒雅致远采纳,获得10
1分钟前
核桃完成签到,获得积分0
1分钟前
排骨大王完成签到,获得积分10
1分钟前
1分钟前
joker完成签到 ,获得积分10
1分钟前
核桃发布了新的文献求助100
1分钟前
1分钟前
温暖元容完成签到,获得积分10
1分钟前
scholar丨崔发布了新的文献求助10
1分钟前
1分钟前
淡定的彩虹完成签到,获得积分10
1分钟前
2分钟前
sss发布了新的文献求助10
2分钟前
2分钟前
情怀应助科研通管家采纳,获得10
2分钟前
2分钟前
卡卡完成签到 ,获得积分10
2分钟前
CNY完成签到 ,获得积分10
2分钟前
赘婿应助_ban采纳,获得10
2分钟前
2分钟前
bksqc发布了新的文献求助10
3分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990020
求助须知:如何正确求助?哪些是违规求助? 3532077
关于积分的说明 11256276
捐赠科研通 3270943
什么是DOI,文献DOI怎么找? 1805139
邀请新用户注册赠送积分活动 882270
科研通“疑难数据库(出版商)”最低求助积分说明 809228