突变体
过氧化物酶体
尿囊素
活性氧
土壤盐分
拟南芥
丙二醛
化学
生物化学
生物
细胞生物学
抗氧化剂
基因
盐度
生态学
作者
Ting Ting Xie,Jiangyu Xu,Wenling Hu,Su-Su Shan,H. Gao,Jiaxin Shen,Xinyi Chen,Yanxiao Jia,Xiuying Gao,Ji Huang,Hongsheng Zhang,Jinping Cheng
摘要
SUMMARY Soil salinization is becoming a great threat that reduces crop productivity worldwide. In this study, we found that rice allantoate amidohydrolase ( OsAAH ) expression was significantly upregulated by salt stress, and its overexpression conferred salt tolerance at the seedling stage. Compared to wild type (WT), the contents of ureides (allantoin and allantoate) were significantly increased in Osaah mutants and reduced in OsAAH overexpression lines both before and after salt treatments. Exogenous allantoin significantly promoted salt tolerance in OsAAH overexpression, but not in Osaah mutants. Subcellular localization showed that OsAAH was also localized to the peroxisomes in addition to the previously reported endoplasmic reticulum (ER). The differential expression of peroxisome‐related genes was identified between Osaah mutants and WT. Furthermore, the contents of H 2 O 2 and malondialdehyde (MDA) were significantly accumulated in Osaah mutants and reduced in OsAAH overexpression lines. The activities of antioxidant enzymes were significantly reduced in Osaah mutants and enhanced in OsAAH overexpression under NaCl treatment. The transcription factor OsABI5 could directly bind to OsAAH promoter and activate OsAAH expression. Our findings reveal that OsAAH could be induced by salt stress through the activation of OsABI5 and then confer salt tolerance by enhancing the scavenging capacity of reactive oxygen species (ROS), which contributes to rice breeding in salt tolerance.
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