微泡
CD44细胞
透明质酸
药物输送
外体
体内
药理学
化学
癌细胞
靶向给药
细胞内
生物相容性
癌症研究
小RNA
体外
癌症
生物化学
医学
生物
生物技术
内科学
有机化学
解剖
基因
作者
Dan Li,Liang Gong,Lin Han,Surui Yao,Yuan Yin,Zhifang Zhou,Jie Shi,Zhimeng Wu,Zhaohui Huang
出处
期刊:Cells
[MDPI AG]
日期:2022-09-29
卷期号:11 (19): 3065-3065
被引量:21
标识
DOI:10.3390/cells11193065
摘要
Cell type-specific drug delivery is a straightforward strategy to achieve targeted cancer therapy and reduce side effects. Hyaluronic acid (HA), an U.S. Food and Drug Administration (FDA)-approved biocompatible carbohydrate polymer, has been extensively employed as a targeting ligand for a drug delivery system due to its natural ability to bind to tumor cells overexpressing cluster of differentiation 44 (CD44) receptors. Here, we report the preparation and antitumor efficacy of HA-coated bovine milk exosomes (HA-mExo) for tumor-specific delivery of microRNA-204-5p mimics (miR-204). The exosome-based delivery formulation was prepared with miR-204 encapsulated inside the lumen and HA displayed outside the membrane. The resultant formulation of HA-mExo-miR204 was able to specifically target CD44-positive cancer cells, with a concomitant increase in the intracellular uptake of miR-204. Compared to the uncoated mExo-miR204 formulation, HA-mExo-miR204 showed significantly increased antitumor efficacy both in vitro and in vivo. Importantly, HA-mExo-miR204 showed excellent biocompatibility and did not cause significant systemic toxicity. Given that both HA and bovine milk exosomes are low-cost and highly accessible biogenic materials with broad biomedical applications, HA-decorated bovine milk exosomes can be proven to be a practical drug delivery system of RNA drugs for targeted cancer therapy.
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