心理压抑
内体
转录因子
二价
细胞内
细胞生物学
突变体
重组DNA
锌指
抄写(语言学)
生物
化学
分子生物学
结合位点
基因
基因表达
生物化学
哲学
语言学
有机化学
作者
Abigail Lapham,Emma Phillips,Chris Barton
出处
期刊:Biochemical Society Transactions
[Portland Press]
日期:2004-10-26
卷期号:32 (6): 1084-1086
被引量:18
摘要
Slc11a1/Nramp1 (solute carrier family 11 member a1/murine natural resistance-associated macrophage protein 1 gene) encodes a divalent cation transporter that resides within lysosomes/late endosomes of macrophages. Nramp1 modulates the cellular distribution of divalent cations in response to cell activation by intracellular pathogens. Nramp1 expression is repressed and activated by the proto-oncogene c-Myc and Miz-1 (c-Myc-interacting zinc finger protein 1) respectively. Here we demonstrate, using a c-Myc mutant (V394D, Val394→Asp) that is incapable of binding Miz-1, that c-Myc repression of Nramp1 transcription is dependent on its interaction with Miz-1. An oligo pull-down assay demonstrates specific binding of recombinant Miz-1 to the Nramp1 Miz-1-binding site or initiator element(s), and Miz-1-dependent c-Myc recruitment.
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