生物
拟南芥
发芽
渗透性休克
花椰菜花叶病毒
脱落酸
转基因作物
互补DNA
非生物胁迫
转基因
基因
基因表达
植物
园艺
生物化学
突变体
作者
Dan Gu,Xihui Liu,Maoyan Wang,Jun Zheng,Wei Hou,Guoying Wang,Jianhua Wang
出处
期刊:Plant Science
[Elsevier]
日期:2007-10-08
卷期号:174 (2): 124-130
被引量:23
标识
DOI:10.1016/j.plantsci.2007.09.010
摘要
12-Oxo-phytodienoic acid reductases (OPRs) are enzymes that catalyze the reduction of double bonds in α,β-unsaturated aldehydes and ketones. In this research, we isolated a full length OPR1 cDNA from Zea mays, which was designated as ZmOPR1. Transcriptional profiling indicated that ZmOPR1 was induced by osmotic stress as well as high salt in the roots of maize seedlings, while the induction of the ZmOPR1 was hardly observed in the leaves. In addition, expression analysis results showed that ZmOPR1 had the highest expression during early seed germination. ABA stress also resulted in a massive accumulation of ZmOPR1 transcripts in both the leaves and roots of maize seedlings. To further explore its biological function, we generated transgenic Arabidopsis plants expressing ZmOPR1 under regulation of the cauliflower mosaic virus CaMV 35S promoter. The transgenic plants exhibited an enhanced level of resistance to osmotic and salt stress during seed germination. Further, RNA gel blot analysis revealed that the expression of ZmOPR1 altered the transcription of some abiotic stress-related genes, including RD22, RD29B and ABF2.
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