荧光
有孔小珠
小分子
化学
分子
转录因子
纳米技术
生物物理学
组合化学
材料科学
生物化学
生物
有机化学
物理
复合材料
基因
量子力学
作者
Margaret Chern,Padric M. Garden,R C. Baer,James E. Galagan,Allison M. Dennis
标识
DOI:10.1002/anie.202007575
摘要
Recently, allosteric transcription factors (TFs) were identified as a novel class of biorecognition elements for in vitro sensing, whereby an indicator of the differential binding affinity between a TF and its cognate DNA exhibits dose-dependent responsivity to an analyte. Described is a modular bead-based biosensor design that can be applied to such TF-DNA-analyte systems. DNA-functionalized beads enable efficient mixing and spatial separation, while TF-labeled semiconductor quantum dots serve as bright fluorescent indicators of the TF-DNA bound (on bead) and unbound states. The prototype sensor for derivatives of the antibiotic tetracycline exhibits nanomolar sensitivity with visual detection of bead fluorescence. Facile changes to the sensor enable sensor response tuning without necessitating changes to the biomolecular affinities. Assay components self-assemble, and readout by eye or digital camera is possible within 5 minutes of analyte addition, making sensor use facile, rapid, and instrument-free.
科研通智能强力驱动
Strongly Powered by AbleSci AI