Highly Sensitive RNA‐Cleaving DNAzyme Sensors from Surface‐to‐Surface Product Enrichment

Abstract The engineering of easy‐to‐use biosensors with ultra‐low detection sensitivity remains a major challenge. Herein, we report a simple approach for creating such sensors through the use of an RNA‐cleaving DNAzyme (RcD) and a strategy designed to concentrate its cleavage product significantly. The assay uses micron‐sized beads loaded with a target‐responsive RcD and a paper strip containing a microzone covered with a DNA oligonucleotide capable of capturing the cleavage product of the RcD through Watson–Crick hybridization. Placing the beads and the paper strip in a target‐containing test sample allows the bead‐bound RcD molecules to undergo target‐induced RNA cleavage, releasing a DNA fragment that is captured by the paper strip. This strategy, though simple, is very effective in achieving high levels of detection sensitivity, being able to enrich the concentration of the cleavage product by three orders of magnitude. It is also compatible with both fluorescence‐based and colorimetric reporting mechanisms. This work provides a simple platform for developing ultrasensitive biosensors that take advantage of the widely available RcDs as molecular recognition elements.