[Qiaoshao Prescription improves premature ejaculation in male rats by increasing the content of 5-hydroxytryptamine].

To observe the intervention effect of Qiaoshao Prescription (QSP) on premature ejaculation (PE) induced by 8-OH-DPAT in male rats and explore its possible action mechanism.Seventy-two male Wistar rats were equally randomized into six groups, blank control, PE model control, low-, medium- and high-dose QSP, and dapoxetine. The PE model was established by injection of 8-OH-DPAT into the subarachnoid space of the lumbosacral spinal cord. Four weeks after modeling, the rats in the blank control and PE model control groups with gavaged with normal saline at 10 ml/kg/d, those in the low-, medium- and high-dose QSP groups with QSP at 5, 10 and 20 g/kg/d respectively once a day, and those in the dapoxetine group with dapoxetine hydrochloride at 300 mg/kg at 3 hours before mating. Forty-five female Wistar rats were injected subcutaneously with 20 μg estradiol benzoate after removal of bilateral ovaries to induce estrous estrus. Two and 4 weeks later, the male rats were mated with the female ones for 30 minutes per time and meanwhile observed for the mating behavior of the males, including mounting latency (ML), intromission latency (IL), ejaculation latency (EL), mounting frequency (MF), intromission frequency (IF), and ejaculation frequency (EF). After the 4th week of mating, the hypothalamus of the animals was isolated and weighed, and the content of 5-hydroxytryptamine (5-HT) was measured.Compared with the blank control group, the PE model controls showed significantly decreased content of 5-HT in the hypothalamus(1 257.1 vs 923.4 ng/g, P<0.05), ML (［11.22 ± 3.60］ vs ［8.69 ± 2.48］ s, P<0.05), IL (［22.33 ± 2.45］ vs ［12.08±1.39］ s, P<0.05), MF (［13.28 ± 3.24］ vs ［7.53 ± 1.84］ times, P<0.05), and EL (［712.35 ± 36.77］ vs ［502.35 ± 46.72］ s, P<0.05). In comparison with the PE model controls, the rats of the QSP and dapoxetine groups exhibited remarkably increased content of 5-HT (P<0.05) and prolonged EL (P<0.05).Qiaoshao Prescription can prolong EL in PE rats, which might be associated with the increased content of 5-HT in the hypothalamus. Further studies, however, are needed on its underlying mechanisms.目的: 观察翘芍方对运用5-羟色胺1A（5-HT1A）受体激动剂8-羟基-2-(二丙基氨基)四氢萘氢溴酸盐（8-OH-DPAT）建立的早泄动物模型的干预效果，并探讨其可能的作用机制。方法: 将雄性Wistar大鼠72只随机分为空白组、模型组、翘芍方组（高、中、低剂量）和达泊西汀组，各12只，以8-OH-DPAT腰骶脊髓节段蛛网膜下腔囊内注射建立早泄动物模型。造模成功后连续给药干预4周，模型组、空白组每天用生理盐水［10 ml/(kg·d)］灌胃；翘芍方低［5g/(kg·d)］、中［10g/(kg·d)］、高［20g/(kg·d)］剂量组，每天灌胃1次；达泊西汀组合笼前3 h给予300 mg/kg盐酸达泊西汀灌胃。雌性Wistar大鼠45只，摘除双侧卵巢后，于合笼前36 h皮下注射苯甲酸雌二醇20μg/只以诱导雌鼠发情。在第2周和第4周时，分别将雄性大鼠和激素诱导的雌性大鼠合笼，进行交配实验，每次30 min。检测指标：观察和记录雄鼠的交配行为，包括骑跨潜伏期(ML)、插入潜伏期(IL)、射精潜伏期(EL)、骑跨次数(MF)、插入次数(IF)、射精次数(EF)。第4周交配试验后，分离出下丘脑并称重，测定5-羟色胺（5-HT）含量。结果： 与空白组相比，模型组5-HT含量923.4 ng/g，ML（8.69±2.48）s、IL（12.08±1.39）s、MF（7.53±1.84）次、EL（502.35±46.72）s分别低于空白组的5-HT含量1 257.1 ng/g，ML（11.22±3.60）s，IL（22.33±2.45）s、MF（13.28±.24）次、EL（712.35±36.77）s，差异均有统计学意义（P＜0.05）；药物干预后，与模型组比较，翘芍方高剂量组和达泊西汀组的5-HT含量均升高，差异有统计学意义（P＜0.05）；与模型组比较，翘芍方各剂量组和达泊西汀组EL明显延长，差异有统计学意义（P＜0.05）。结论： 翘芍方可延长早泄大鼠的射精潜伏期，这可能与其增加了下丘脑5-HT含量有关，具体机制还需进一步研究。