Two bi‐functional cytochrome P450 CYP72 enzymes from olive ( Olea europaea ) catalyze the oxidative C‐C bond cleavage in the biosynthesis of secoxy‐iridoids – flavor and quality determinants in olive oil

橄榄油 生物化学 生物 细胞色素P450 植物
作者
Carlos E. Rodríguez López,Benke Hong,Christian Paetz,Yoko Nakamura,Κωνσταντίνος Κουδούνας,Valentina Passeri,Luciana Baldoni,Fiammetta Alagna,Ornella Calderini,Sarah E. O’Connor
出处
期刊:New Phytologist [Wiley]
卷期号:229 (4): 2288-2301 被引量:19
标识
DOI:10.1111/nph.16975
摘要

Summary Olive ( Olea europaea ) is an important crop in Europe, with high cultural, economic and nutritional significance. Olive oil flavor and quality depend on phenolic secoiridoids, but the biosynthetic pathway of these iridoids remains largely uncharacterized. We discovered two bifunctional cytochrome P450 enzymes, catalyzing the rare oxidative C‐C bond cleavage of 7‐ epi ‐loganin to produce oleoside methyl ester (OeOMES) and secoxyloganin (OeSXS), both through a ketologanin intermediary. Although these enzymes are homologous to the previously reported Catharanthus roseus secologanin synthase (CrSLS), the substrate and product profiles differ. Biochemical assays provided mechanistic insights into the two‐step OeOMES and CrSLS reactions. Model‐guided mutations of OeOMES changed the product profile in a predictable manner, revealing insights into the molecular basis for this change in product specificity. Our results suggest that, in contrast to published hypotheses, in planta production of secoxy‐iridoids is secologanin‐independent. Notably, sequence data of cultivated and wild olives point to a relation between domestication and OeOMES expression. Thus, the discovery of this key biosynthetic gene suggests a link between domestication and secondary metabolism, and could potentially be used as a genetic marker to guide next‐generation breeding programs.
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