Native high-resolution mass spectrometry analysis of noncovalent protein complexes up to 450 kDa

BioanalysisVol. 12, No. 19 MethodologyNative high-resolution mass spectrometry analysis of noncovalent protein complexes up to 450 kDaZhuo Chen, John F Kellie, Charles S Hottenstein & Matthew E SzapacsZhuo Chen*Author for correspondence: Tel.: +1 610 917 2846; Fax: +1 610 270 5604; E-mail Address: zhuo.2.chen@gsk.comBioanalysis, Immunogenicity & Biomarkers, In-Vitro/In-Vivo Translation, R&D Research, GlaxoSmithKline Pharmaceuticals, 1250 South Collegeville Rd, Collegeville, PA 19426, USASearch for more papers by this author, John F KellieBioanalysis, Immunogenicity & Biomarkers, In-Vitro/In-Vivo Translation, R&D Research, GlaxoSmithKline Pharmaceuticals, 1250 South Collegeville Rd, Collegeville, PA 19426, USASearch for more papers by this author, Charles S HottensteinBioanalysis, Immunogenicity & Biomarkers, In-Vitro/In-Vivo Translation, R&D Research, GlaxoSmithKline Pharmaceuticals, 1250 South Collegeville Rd, Collegeville, PA 19426, USASearch for more papers by this author & Matthew E SzapacsBioanalysis, Immunogenicity & Biomarkers, In-Vitro/In-Vivo Translation, R&D Research, GlaxoSmithKline Pharmaceuticals, 1250 South Collegeville Rd, Collegeville, PA 19426, USASearch for more papers by this authorPublished Online:24 Aug 2020https://doi.org/10.4155/bio-2020-0145AboutSectionsView ArticleView Full TextPDF/EPUB ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareShare onFacebookTwitterLinkedInReddit View articleKeywords: native mass spectrometrynon-covalent protein complextarget engagementPapers of special note have been highlighted as: • of interest; •• of considerable interestReferences1. 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J. 81(6), 3503–3509 (2001).Crossref, Medline, CAS, Google ScholarFiguresReferencesRelatedDetailsCited ByDetermination of label efficiency and label degree of critical reagents by LC-MS and native MSAnalytical Biochemistry, Vol. 664Approaches to Heterogeneity in Native Mass Spectrometry2 September 2021 | Chemical Reviews, Vol. 122, No. 8Thermodynamic Evaluation of the Interactions between Anticancer Pt(II) Complexes and Model Proteins19 April 2021 | Molecules, Vol. 26, No. 8 Vol. 12, No. 19 Follow us on social media for the latest updates Metrics Downloaded 231 times History Received 27 May 2020 Accepted 27 July 2020 Published online 24 August 2020 Published in print October 2020 Information© 2020 Newlands PressKeywordsnative mass spectrometrynon-covalent protein complextarget engagementAcknowledgmentsThe authors gratefully appreciate the edits and suggestions on manuscript from T Sikorski. The authors also acknowledge C Evans and E Yang for supporting this research.Financial & competing interests disclosureThis research was supported by GlaxoSmithKline Research and Development. All authors are employees of GlaxoSmithKline, whom may be eligible for stock options or have stock ownership. The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed.No writing assistance was utilized in the production of this manuscript.Ethical conduct of researchThe authors state that they have obtained appropriate institutional review board approval or have followed the principles outlined in the Declaration of Helsinki for all human or animal experimental investigations. In addition, for investigations involving human subjects, informed consent has been obtained from the participants involved.PDF download