杂种优势
遗传学
生物
基因
鉴定(生物学)
候选基因
计算生物学
生物信息学
混合的
植物
作者
R C Venu,Jianbing Ma,Yulin Jia,Guangjie Liu,Melissa H. Jia,Kan Nobuta,M V Sreerekha,K. A. K. Moldenhauer,Anna M. McClung,Blake C. Meyers,Guo‐Liang Wang
出处
期刊:PLOS ONE
[Public Library of Science]
日期:2014-04-17
卷期号:9 (4): e95178-e95178
被引量:14
标识
DOI:10.1371/journal.pone.0095178
摘要
To identify the genes responsible for yield related traits, and heterosis, massively parallel signature sequencing (MPSS) libraries were constructed from leaves, roots and meristem tissues from the two parents, 'Nipponbare' and '93-11', and their F1 hybrid. From the MPSS libraries, 1-3 million signatures were obtained. Using cluster analysis, commonly and specifically expressed genes in the parents and their F1 hybrid were identified. To understand heterosis in the F1 hybrid, the differentially expressed genes in the F1 hybrid were mapped to yield related quantitative trait loci (QTL) regions using a linkage map constructed from 131 polymorphic simple sequence repeat markers with 266 recombinant inbred lines derived from a cross between Nipponbare and 93-11. QTLs were identified for yield related traits including days to heading, plant height, plant type, number of tillers, main panicle length, number of primary branches per main panicle, number of kernels per main panicle, total kernel weight per main panicle, 1000 grain weight and total grain yield per plant. Seventy one QTLs for these traits were mapped, of which 3 QTLs were novel. Many highly expressed chromatin-related genes in the F1 hybrid encoding histone demethylases, histone deacetylases, argonaute-like proteins and polycomb proteins were located in these yield QTL regions. A total of 336 highly expressed transcription factor (TF) genes belonging to 50 TF families were identified in the yield QTL intervals. These findings provide the starting genomic materials to elucidate the molecular basis of yield related traits and heterosis in rice.
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