双环分子
噬菌体展示
肽库
肽
组合化学
计算生物学
配体(生物化学)
选择(遗传算法)
化学
生物
生物化学
立体化学
计算机科学
肽序列
受体
基因
机器学习
作者
Inmaculada Rentero Rebollo,Christian Heinis
出处
期刊:Methods
[Elsevier]
日期:2013-03-01
卷期号:60 (1): 46-54
被引量:63
标识
DOI:10.1016/j.ymeth.2012.12.008
摘要
Bicyclic peptides are small, constrained peptides that can bind with high affinity and selectivity to protein targets. Their small size provides a number of advantages over larger protein-based ligands, including access to chemical synthesis, better tissue penetration, and a wider choice of application routes. Bicyclic peptide ligands can be identified using phage display technology with moderate effort and cost. Here we provide step-by-step protocols for the isolation of bicyclic peptide ligands using phage display. These protocols have been successfully used in our laboratory for the generation of high-affinity binders to a variety of protein targets. We describe library generation, affinity selection and ligand characterization, and provide troubleshooting advice concerning frequent problems.
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