Callus formation in ferula jaeschkeana vatke

Callus was initiated from the petiole explants of Ferula jaeschakeana on a medium containing 2,4-D.The highest percentage of explants forming callus (92.5) and callus index (258) were obtained on medium supplemented with 1 mgl -l 2,4-D.Among explants sizes and type tested for callus initiation, 10 mm long petiole gave maximum callus index.Small and large explants initiated a slight amount of callus.Addition of Kn in combination with 2,4-D enhanced the callus formation from the explants.