腰果内酯
糖苷
生物测定
洋地黄素
柱色谱法
色谱法
化学
高效液相色谱法
天然产物
葡萄糖苷
立体化学
植物
生物
病理
替代医学
医学
遗传学
作者
Mark S. Butler,Leanne Towerzey,Ngoc Bich Pham,Edward Hyde,Sao Khemar Wadi,Gordon P. Guymer,Ronald J. Quinn
标识
DOI:10.1016/j.phytochem.2013.11.024
摘要
Extracts from dried leaf and stems of Elaeodendron australe var. integrifolium (Celastraceae) collected in South East Queensland, Australia, were active in an assay that measured Ca2+ driven expression of IL-2/luciferase designed to identify inhibitors of the ICRAC channel. Bioassay-guided isolation using C18 and polyamide column chromatography, HPLC (Phenyl and C18) and centrifugal partition chromatography (CPC) led to the isolation of digitoxigenin (1) and three cardenolide glycosides, glucoside 2, quinovoside 3 and the new natural product xyloside 4, as the active components with low nM activity in the reporter assay.
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