Abstract A floret carries only one pistil that will develop into one grain after fertilization in normal cultivated common wheat ( Triticum aestivum L.) plants. A new cultivated common wheat mutation line ‘Three Pistils’ is described. It carries three pistils in a floret, all with the potential to develop into grains. The floret morphology of this line is reported. Genetic analysis of the three pistils trait was carried out by crossing Three Pistils with the normal common wheat variety Chinese Spring. F 2 population segregation analysis revealed that the three pistils trait is controlled by a single dominant gene. This conclusion was confirmed by the backcross test.