拟南芥
生物
基因
基因表达
生物钟
突变体
基因表达谱
遗传学
拟南芥
细胞生物学
昼夜节律
内分泌学
作者
Norihito Nakamichi,Miyako Kusano,Atsushi Fukushima,Masanori Kita,Shogo Ito,Takafumi Yamashino,Kazuki Saito,Hitoshi Sakakibara,Takeshi Mizuno
出处
期刊:Plant and Cell Physiology
[Oxford University Press]
日期:2009-01-08
卷期号:50 (3): 447-462
被引量:273
摘要
Arabidopsis PSEUDO RESPONSE REGULATOR (PRR) genes are components of the circadian clock mechanism. In order to understand the scope of genome-wide transcriptional regulation by PRR genes, a comparison survey of gene expression in wild-type Arabidopsis and a prr9-11 prr7-10 prr5-10 triple mutant (d975) using mRNA collected during late daytime was conducted using an Affymetrix ATH-1 GeneChip®. The expression of ‘night genes’ increased and the expression of ‘day genes’ decreased toward the end of the diurnal light phase, but expression of these genes was essentially constant in d975. The expression levels of ‘night genes’ were lower, whereas the expression of ‘day genes’ was higher in d975 than in the wild type. Bioinformatics approaches have indicated that the set of up-regulated genes in d975 and the set of cold-responsive genes have significant overlap. We found that d975 is more tolerant to cold, high salinity and drought stresses than the wild type. In addition, dehydration-responsive element B1/C-repeat-binding factor (DREB1/CBF), which is expressed around mid-day, is more highly expressed in d975. Raffinose and l-proline accumulated at higher levels in d975 even when plants were grown under normal conditions. These results suggest that PRR9, PRR7 and PRR5 are involved in a mechanism that anticipates diurnal cold stress and which initiates a stress response by mediating cyclic expression of stress response genes, including DREB1/CBF.
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