蛋白质组
单核细胞增生李斯特菌
拉伤
蛋白质组学
生物
生物膜
转录组
细菌
微生物学
单元格信封
李斯特菌
细胞生物学
遗传学
大肠杆菌
基因
基因表达
解剖
作者
Márcia Magalhães Mata,Wladimir Padilha da Silva,Richard Wilson,Edwin K. Lowe,John P. Bowman
摘要
Contamination of industrial and domestic food usage environments by the attachement of bacterial food-borne pathogen Listeria monocytogenes has public health and economic implications. Comprehensive proteomics experiments using label-free liquid chromatography/tandem mass spectrometry were used to compare the proteomes of two different L. monocytogenes strains (Siliken_1/2c and F2365_4b), which show very different capacities to attach to surfaces. Growth temperature and strain type were highly influential on the proteomes in both attached and planktonic cells. On the basis of the proteomic data, it is highly unlikely that specific surface proteins play a direct role in adherence to inanimate surfaces. Instead, strain-dependent responses related to cell envelope polymer biosynthesis and stress response regulation likely contribute to a different ability to attach and also to survive external stressors. Collectively, the divergent proteome-level responses observed define strain- and growth-temperature-dependent differences relevant to attachment efficacy, highlight relevant proteins involved in stress protection in attached cells, and suggest that strain differences and growth conditions are important in relation to environmental persistence.
科研通智能强力驱动
Strongly Powered by AbleSci AI