Influence of phytohormones and medium on the shoot regeneration from leaf of Swertia chirata Buch.-Ham. Ex wall. in vitro

A procedure for in vitro shoot regeneration was formed from leaves derived from the field grown Swertia chirata and the effects of phytohormones and medium on the regeneration of shoot were tested by an orthogonal experiments. The best result was obtained in the 190-2 medium with 13.32 mu M 6-benzylaminopurine and 0.54 mu M alpha-naphthaleneacetic acid. The result of orthogonal analysis of variance showed that 6-benzylaminopurine and alpha-naphthaleneacetic acid significantly affected on the shoot regeneration. The propagation rate was 7.75 +/- 3.46 when the shoots were cultured on the multiplication medium. The highest rate of shoots was 96.50% on the medium with 5.40 mu M alpha-naphthaleneacetic acid. Histological studies showed shoot regeneration took place by somatic embryogenesis.