生物
染色质
增强子
基因组
基因
DNA
抄写(语言学)
CpG站点
转录因子
DNA病毒
病毒学
遗传学
细胞生物学
基因表达
DNA甲基化
哲学
语言学
作者
Pierrick Moreau,Axel Cournac,G.A. Palumbo,Martial Marbouty,Shogofa Mortaza,Agnès Thierry,Stefano Cairo,Marc Lavigne,Romain Koszul,Christine Neuveut
标识
DOI:10.1038/s41467-018-06739-4
摘要
Abstract Whether non-integrated viral DNAs distribute randomly or target specific positions within the higher-order architecture of mammalian genomes remains largely unknown. Here we use Hi-C and viral DNA capture (CHi-C) in primary human hepatocytes infected by either hepatitis B virus (HBV) or adenovirus type 5 (Ad5) virus to show that they adopt different strategies in their respective positioning at active chromatin. HBV contacts preferentially CpG islands (CGIs) enriched in Cfp1 a factor required for its transcription. These CGIs are often associated with highly expressed genes (HEG) and genes deregulated during infection. Ad5 DNA interacts preferentially with transcription start sites (TSSs) and enhancers of HEG, as well as genes upregulated during infection. These results show that DNA viruses use different strategies to infiltrate genomic 3D networks and target specific regions. This targeting may facilitate the recruitment of transcription factors necessary for their own replication and contribute to the deregulation of cellular gene expression.
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