CYP46A1 gene therapy deciphers the role of brain cholesterol metabolism in Huntington’s disease

亨廷顿蛋白 亨廷顿病 神经保护 生物 亨廷顿蛋白 神经科学 细胞生物学 内科学 医学 疾病
作者
Radhia Kacher,Antonin Lamazière,Nicolas Heck,Vincent Kappès,Coline Mounier,Gaëtan Déspres,Yulia Dembitskaya,Elodie Perrin,Wilhelm Christaller,Satish S. Nair,Valérie Grange‐Messent,Nathalie Cartier,Peter Vanhoutte,Laurent Venance,Frédéric Saudou,Christian Néri,Jocelyne Caboche,Sandrine Bétuing
出处
期刊:Brain [Oxford University Press]
卷期号:142 (8): 2432-2450 被引量:85
标识
DOI:10.1093/brain/awz174
摘要

Abstract Dysfunctions in brain cholesterol homeostasis have been extensively related to brain disorders. The main pathway for brain cholesterol elimination is its hydroxylation into 24S-hydroxycholesterol by the cholesterol 24-hydrolase, CYP46A1. Increasing evidence suggests that CYP46A1 has a role in the pathogenesis and progression of neurodegenerative disorders, and that increasing its levels in the brain is neuroprotective. However, the mechanisms underlying this neuroprotection remain to be fully understood. Huntington’s disease is a fatal autosomal dominant neurodegenerative disease caused by an abnormal CAG expansion in huntingtin’s gene. Among the multiple cellular and molecular dysfunctions caused by this mutation, altered brain cholesterol homeostasis has been described in patients and animal models as a critical event in Huntington’s disease. Here, we demonstrate that a gene therapy approach based on the delivery of CYP46A1, the rate-limiting enzyme for cholesterol degradation in the brain, has a long-lasting neuroprotective effect in Huntington’s disease and counteracts multiple detrimental effects of the mutated huntingtin. In zQ175 Huntington’s disease knock-in mice, CYP46A1 prevented neuronal dysfunctions and restored cholesterol homeostasis. These events were associated to a specific striatal transcriptomic signature that compensates for multiple mHTT-induced dysfunctions. We thus explored the mechanisms for these compensations and showed an improvement of synaptic activity and connectivity along with the stimulation of the proteasome and autophagy machineries, which participate to the clearance of mutant huntingtin (mHTT) aggregates. Furthermore, BDNF vesicle axonal transport and TrkB endosome trafficking were restored in a cellular model of Huntington’s disease. These results highlight the large-scale beneficial effect of restoring cholesterol homeostasis in neurodegenerative diseases and give new opportunities for developing innovative disease-modifying strategies in Huntington’s disease.

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