已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

Tyro3, Axl, and Mertk receptors differentially participate in platelet activation and thrombus formation

梅尔特克 气体6 血小板活化 血栓 受体 血小板 传出细胞增多 细胞生物学 化学 癌症研究 医学 免疫学 生物 内科学 生物化学 受体酪氨酸激酶 巨噬细胞 体外
作者
Junsong Zhou,Aizhen Yang,Yucan Wang,Hao Chen,Zhenzhen Zhao,Viralkumar Davra,Katsue Suzuki‐Inoue,Yukio Ozaki,Raymond B. Birge,Qingxian Lu,Yi Wu
出处
期刊:Cell Communication and Signaling [Springer Nature]
卷期号:16 (1) 被引量:25
标识
DOI:10.1186/s12964-018-0308-0
摘要

Previously, several studies have shown that Tyro3, Axl, and Mertk (TAM) receptors participate in platelet activation and thrombosis. However, the role of individual receptors is not fully understood. Using single receptor-deficient platelets from TAM knockout mice in the C57BL/6 J strain, we performed a knockout study using single TAM-deficient mice. We treated platelets isolated from TAM knockout mice with the Glycoprotein VI (GPVI) agonists convulxin, poly(PHG), and collagen-related triple-helical peptide (CRP), as well as thrombin for in-vitro experiments. We used a laser-induced cremaster arterial injury model for thrombosis experiments in vivo. Deficiency of the tyrosine kinase receptors, Axl or Tyro3, but not Mertk, inhibited aggregation, spreading, JON/A binding, and P-selectin expression of platelets in vitro. In vivo, platelet thrombus formation was significantly decreased in Axl−/− and Tyro3−/− mice, but not in Mertk−/− mice. Upon stimulation with glycoprotein VI (GPVI) agonists, tyrosine phosphorylation of signaling molecules, including spleen tyrosine kinase (Syk) and phospholipase C-γ2 (PLCγ2), was decreased in Axl−/− and Tyro3−/− platelets, but not in Mertk−/− platelets. While platelet aggregation induced by agonists did not differ in the presence or absence of the Gas6 neutralizing antibody, the platelet aggregation was inhibited by anti-Axl or anti-Tyro3 neutralizing antibodies antibody, but not the anti-Mertk antibody. Additionally, the recombinant extracellular domain of Axl or Tyro3, but not that of Mertk, also inhibited platelet aggregation. These data suggest that Axl and Tyro3, but not Mertk, have an important role in platelet activation and thrombus formation, and mechanistically may do so by a pathway that regulates inside to outside signaling and heterotypic interactions via the extracellular domains of TAMs.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
2秒前
Jasper应助果果采纳,获得10
3秒前
4秒前
诚心的信封完成签到 ,获得积分10
5秒前
喵呜啦啦啦啦完成签到,获得积分10
6秒前
哈哈哈完成签到,获得积分10
6秒前
文艺以莲发布了新的文献求助10
7秒前
ifegiugfieugfig完成签到,获得积分10
9秒前
12秒前
义气珩发布了新的文献求助10
15秒前
沈婉婉完成签到 ,获得积分10
17秒前
18秒前
fuueer完成签到 ,获得积分10
18秒前
OIIII完成签到 ,获得积分10
18秒前
果果发布了新的文献求助10
19秒前
六六发布了新的文献求助10
23秒前
24秒前
25秒前
甜蜜的无声完成签到 ,获得积分10
25秒前
jeff发布了新的文献求助10
29秒前
31秒前
义气珩完成签到,获得积分10
33秒前
酷波er应助文艺以莲采纳,获得10
35秒前
赘婿应助心灵美的南晴采纳,获得10
37秒前
啦啦啦完成签到 ,获得积分10
39秒前
42秒前
xu完成签到,获得积分20
43秒前
文艺以莲完成签到,获得积分10
45秒前
xu发布了新的文献求助10
46秒前
香蕉觅云应助xu采纳,获得10
1分钟前
1分钟前
Xielin完成签到,获得积分10
1分钟前
1分钟前
1分钟前
惑感完成签到 ,获得积分10
1分钟前
bzlinhqu@126.com完成签到 ,获得积分10
1分钟前
扎菜发布了新的文献求助10
1分钟前
爱静静应助hata233采纳,获得10
1分钟前
NexusExplorer应助Sophist采纳,获得10
1分钟前
1分钟前
高分求助中
Earth System Geophysics 1000
Co-opetition under Endogenous Bargaining Power 666
Medicina di laboratorio. Logica e patologia clinica 600
Sarcolestes leedsi Lydekker, an ankylosaurian dinosaur from the Middle Jurassic of England 500
《关于整治突出dupin问题的实施意见》(厅字〔2019〕52号) 500
Language injustice and social equity in EMI policies in China 500
mTOR signalling in RPGR-associated Retinitis Pigmentosa 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3213078
求助须知:如何正确求助?哪些是违规求助? 2861888
关于积分的说明 8130903
捐赠科研通 2527823
什么是DOI,文献DOI怎么找? 1361739
科研通“疑难数据库(出版商)”最低求助积分说明 643516
邀请新用户注册赠送积分活动 615861