Embryonic stem cell- and transcriptomics-based in vitro analyses reveal that bisphenols A, F and S have similar and very complex potential developmental toxicities

胚胎干细胞 生物 干细胞 转录组 祖细胞 胚状体 细胞生物学 发育毒性 神经干细胞 细胞分化 诱导多能干细胞 遗传学 基因 基因表达 妊娠期 怀孕
作者
Nuoya Yin,Xiaoxing Liang,Shengxian Liang,Siwei Liang,Renjun Yang,Bowen Hu,Zhanwen Cheng,Shuyu Liu,Hengzhi Dong,Sijin Liu,Francesco Faiola
出处
期刊:Ecotoxicology and Environmental Safety [Elsevier]
卷期号:176: 330-338 被引量:46
标识
DOI:10.1016/j.ecoenv.2019.03.115
摘要

Bisphenol A (BPA) is a very versatile industrial chemical. Many reports have associated BPA with several health effects. Some bisphenol alternatives have been introduced to replace BPA in its many applications. However, comprehensive toxicological evaluations for these replacements are still lacking. In this study, we examined the potential effects of BPA, bisphenol F (BPF) and bisphenol S (BPS), on embryonic development with an in vitro stem cell toxicology system and transcriptomics analyses. Mouse embryonic stem cells (mESCs) were differentiated via embryoid body formation, either globally towards the three primary germ layers and their lineages, or specifically into neuroectoderm/neural progenitor cells. During the differentiation, cells were treated with BPA, BPF, BPS, or DMSO control. Samples were collected at different time points, for qRT-PCR and RNA-seq analyses. BPA, BPF and BPS disrupted many processes, during mESC global and neural differentiations, in very similar manners. In fact, at each time point the three chemicals differentially regulated analogous gene categories, particularly the ones involved in cell-matrix and cell-cell adhesion, signal transduction pathways, and medical conditions such as cardiovascular diseases and cancer. Our findings demonstrate once more then BPA substitutes may not be very safe. They potentially have a very complex developmental toxicity, similarly to BPA, and seem more toxic than BPA itself. In addition, our results reveal that stem cell-based developmental toxicity assays can be very comprehensive.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
西瓜完成签到 ,获得积分10
2秒前
JamesPei应助李亨达采纳,获得10
8秒前
JamesPei应助科研通管家采纳,获得10
14秒前
14秒前
李亨达完成签到,获得积分20
22秒前
22秒前
李亨达发布了新的文献求助10
26秒前
30秒前
leilani完成签到 ,获得积分10
32秒前
文在否完成签到 ,获得积分10
34秒前
Jolleyhaha完成签到 ,获得积分10
38秒前
czj完成签到 ,获得积分10
41秒前
落后的皮卡丘完成签到,获得积分10
42秒前
隐形故事完成签到,获得积分20
52秒前
春色未软旧苔痕完成签到 ,获得积分10
59秒前
yuminger完成签到 ,获得积分10
1分钟前
1分钟前
是小小李哇完成签到 ,获得积分10
1分钟前
隐形故事关注了科研通微信公众号
1分钟前
bluesiryao发布了新的文献求助10
1分钟前
负责冰海完成签到 ,获得积分10
1分钟前
称心采枫完成签到 ,获得积分10
1分钟前
meijuan1210完成签到 ,获得积分10
1分钟前
1分钟前
1分钟前
Ryan完成签到 ,获得积分10
1分钟前
桂花完成签到 ,获得积分10
1分钟前
牛轧糖完成签到 ,获得积分10
1分钟前
工藤新一完成签到 ,获得积分10
1分钟前
大咖完成签到 ,获得积分10
2分钟前
logolush完成签到 ,获得积分10
2分钟前
Singularity应助abc采纳,获得10
2分钟前
2分钟前
萧水白应助科研通管家采纳,获得10
2分钟前
nicheng完成签到 ,获得积分0
2分钟前
keyan123完成签到,获得积分10
2分钟前
科研通AI2S应助ydz采纳,获得10
2分钟前
晚街听风完成签到 ,获得积分10
2分钟前
Coffey完成签到 ,获得积分10
2分钟前
科研通AI2S应助ydz采纳,获得10
2分钟前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Evolution 3rd edition 1500
保险藏宝图 1000
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 700
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 700
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3186894
求助须知:如何正确求助?哪些是违规求助? 2836960
关于积分的说明 8012007
捐赠科研通 2499375
什么是DOI,文献DOI怎么找? 1334354
科研通“疑难数据库(出版商)”最低求助积分说明 637170
邀请新用户注册赠送积分活动 605071