Embryonic stem cell- and transcriptomics-based in vitro analyses reveal that bisphenols A, F and S have similar and very complex potential developmental toxicities

胚胎干细胞 生物 干细胞 转录组 祖细胞 胚状体 细胞生物学 发育毒性 神经干细胞 细胞分化 诱导多能干细胞 遗传学 基因 基因表达 妊娠期 怀孕
作者
Nuoya Yin,Xiaoxing Liang,Shengxian Liang,Siwei Liang,Renjun Yang,Bowen Hu,Zhanwen Cheng,Shuyu Liu,Hengzhi Dong,Sijin Liu,Francesco Faiola
出处
期刊:Ecotoxicology and Environmental Safety [Elsevier]
卷期号:176: 330-338 被引量:46
标识
DOI:10.1016/j.ecoenv.2019.03.115
摘要

Bisphenol A (BPA) is a very versatile industrial chemical. Many reports have associated BPA with several health effects. Some bisphenol alternatives have been introduced to replace BPA in its many applications. However, comprehensive toxicological evaluations for these replacements are still lacking. In this study, we examined the potential effects of BPA, bisphenol F (BPF) and bisphenol S (BPS), on embryonic development with an in vitro stem cell toxicology system and transcriptomics analyses. Mouse embryonic stem cells (mESCs) were differentiated via embryoid body formation, either globally towards the three primary germ layers and their lineages, or specifically into neuroectoderm/neural progenitor cells. During the differentiation, cells were treated with BPA, BPF, BPS, or DMSO control. Samples were collected at different time points, for qRT-PCR and RNA-seq analyses. BPA, BPF and BPS disrupted many processes, during mESC global and neural differentiations, in very similar manners. In fact, at each time point the three chemicals differentially regulated analogous gene categories, particularly the ones involved in cell-matrix and cell-cell adhesion, signal transduction pathways, and medical conditions such as cardiovascular diseases and cancer. Our findings demonstrate once more then BPA substitutes may not be very safe. They potentially have a very complex developmental toxicity, similarly to BPA, and seem more toxic than BPA itself. In addition, our results reveal that stem cell-based developmental toxicity assays can be very comprehensive.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
隐形世开发布了新的文献求助10
1秒前
庆次完成签到 ,获得积分10
1秒前
林夏发布了新的文献求助10
1秒前
isvv完成签到,获得积分20
1秒前
chali48完成签到 ,获得积分10
1秒前
WY-zicaitang发布了新的文献求助10
3秒前
taozidetao发布了新的文献求助10
3秒前
标致乐双完成签到,获得积分10
8秒前
迅速泽洋完成签到,获得积分10
8秒前
9秒前
我是老大应助明亮无颜采纳,获得30
10秒前
斯文败类应助天天天晴采纳,获得10
10秒前
WY-zicaitang完成签到,获得积分10
11秒前
山海之间完成签到,获得积分10
11秒前
杳鸢应助务实小鸽子采纳,获得10
11秒前
梧桐完成签到,获得积分10
11秒前
不配.给leungya的求助进行了留言
13秒前
九零后无心完成签到,获得积分10
13秒前
13秒前
梧桐发布了新的文献求助10
13秒前
14秒前
14秒前
内向雅香发布了新的文献求助10
14秒前
15秒前
15秒前
17秒前
18秒前
Chocolat_Chaud完成签到 ,获得积分10
19秒前
19秒前
19秒前
taozi完成签到,获得积分10
20秒前
mignan发布了新的文献求助10
20秒前
21秒前
bkagyin应助阳光依玉采纳,获得10
22秒前
LSY完成签到,获得积分10
22秒前
23秒前
汤汤发布了新的文献求助10
25秒前
完美世界应助GXC0304采纳,获得10
26秒前
jia完成签到 ,获得积分10
26秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Evolution 3rd edition 1500
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 700
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 700
ALA生合成不全マウスでの糖代謝異常の分子機構解析 520
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3187416
求助须知:如何正确求助?哪些是违规求助? 2837320
关于积分的说明 8014314
捐赠科研通 2499932
什么是DOI,文献DOI怎么找? 1334645
科研通“疑难数据库(出版商)”最低求助积分说明 637200
邀请新用户注册赠送积分活动 605182