Embryonic stem cell- and transcriptomics-based in vitro analyses reveal that bisphenols A, F and S have similar and very complex potential developmental toxicities

胚胎干细胞 生物 干细胞 转录组 祖细胞 胚状体 细胞生物学 发育毒性 神经干细胞 细胞分化 诱导多能干细胞 遗传学 基因 基因表达 妊娠期 怀孕
作者
Nuoya Yin,Xiaoxing Liang,Shengxian Liang,Siwei Liang,Renjun Yang,Bowen Hu,Zhanwen Cheng,Shuyu Liu,Hengzhi Dong,Sijin Liu,Francesco Faiola
出处
期刊:Ecotoxicology and Environmental Safety [Elsevier]
卷期号:176: 330-338 被引量:46
标识
DOI:10.1016/j.ecoenv.2019.03.115
摘要

Bisphenol A (BPA) is a very versatile industrial chemical. Many reports have associated BPA with several health effects. Some bisphenol alternatives have been introduced to replace BPA in its many applications. However, comprehensive toxicological evaluations for these replacements are still lacking. In this study, we examined the potential effects of BPA, bisphenol F (BPF) and bisphenol S (BPS), on embryonic development with an in vitro stem cell toxicology system and transcriptomics analyses. Mouse embryonic stem cells (mESCs) were differentiated via embryoid body formation, either globally towards the three primary germ layers and their lineages, or specifically into neuroectoderm/neural progenitor cells. During the differentiation, cells were treated with BPA, BPF, BPS, or DMSO control. Samples were collected at different time points, for qRT-PCR and RNA-seq analyses. BPA, BPF and BPS disrupted many processes, during mESC global and neural differentiations, in very similar manners. In fact, at each time point the three chemicals differentially regulated analogous gene categories, particularly the ones involved in cell-matrix and cell-cell adhesion, signal transduction pathways, and medical conditions such as cardiovascular diseases and cancer. Our findings demonstrate once more then BPA substitutes may not be very safe. They potentially have a very complex developmental toxicity, similarly to BPA, and seem more toxic than BPA itself. In addition, our results reveal that stem cell-based developmental toxicity assays can be very comprehensive.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
曲奇饼干完成签到,获得积分10
1秒前
2秒前
空城完成签到,获得积分10
2秒前
尛瞐慶成发布了新的文献求助10
3秒前
Ridley发布了新的文献求助10
3秒前
二月why完成签到,获得积分10
3秒前
4秒前
4秒前
金玉完成签到,获得积分10
5秒前
奋斗的板栗完成签到,获得积分10
5秒前
6秒前
6秒前
6秒前
安详流沙发布了新的文献求助10
6秒前
wen完成签到,获得积分10
7秒前
Kirito发布了新的文献求助30
8秒前
Jasper应助无情的盼兰采纳,获得10
9秒前
搜集达人应助科研通管家采纳,获得10
9秒前
彭于晏应助科研通管家采纳,获得10
9秒前
9秒前
大模型应助科研通管家采纳,获得20
9秒前
Lucas应助科研通管家采纳,获得10
9秒前
leec发布了新的文献求助10
10秒前
10秒前
10秒前
天真的不尤完成签到 ,获得积分10
12秒前
执着的妙芙应助渤海少年采纳,获得10
13秒前
义气尔安完成签到,获得积分10
13秒前
13秒前
汉堡包应助lala采纳,获得10
14秒前
苹果柚子完成签到,获得积分10
14秒前
852应助安详流沙采纳,获得10
15秒前
amo完成签到,获得积分10
16秒前
17秒前
17秒前
爆米花应助tomorrow采纳,获得10
18秒前
33完成签到,获得积分20
19秒前
dawei完成签到 ,获得积分10
19秒前
21秒前
小张早日毕业完成签到,获得积分10
21秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
ALA生合成不全マウスでの糖代謝異常の分子機構解析 520
Aspects of Babylonian celestial divination: the lunar eclipse tablets of Enūma Anu Enlil 500
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
2024 Medicinal Chemistry Reviews 400
Dictionary of socialism 350
Mixed-anion Compounds 300
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3197965
求助须知:如何正确求助?哪些是违规求助? 2846805
关于积分的说明 8060914
捐赠科研通 2511732
什么是DOI,文献DOI怎么找? 1343641
科研通“疑难数据库(出版商)”最低求助积分说明 639600
邀请新用户注册赠送积分活动 609229