Embryonic stem cell- and transcriptomics-based in vitro analyses reveal that bisphenols A, F and S have similar and very complex potential developmental toxicities

胚胎干细胞 生物 干细胞 转录组 祖细胞 胚状体 细胞生物学 发育毒性 神经干细胞 细胞分化 诱导多能干细胞 遗传学 基因 基因表达 妊娠期 怀孕
作者
Nuoya Yin,Xiaoxing Liang,Shengxian Liang,Siwei Liang,Renjun Yang,Bowen Hu,Zhanwen Cheng,Shuyu Liu,Hengzhi Dong,Sijin Liu,Francesco Faiola
出处
期刊:Ecotoxicology and Environmental Safety [Elsevier]
卷期号:176: 330-338 被引量:46
标识
DOI:10.1016/j.ecoenv.2019.03.115
摘要

Bisphenol A (BPA) is a very versatile industrial chemical. Many reports have associated BPA with several health effects. Some bisphenol alternatives have been introduced to replace BPA in its many applications. However, comprehensive toxicological evaluations for these replacements are still lacking. In this study, we examined the potential effects of BPA, bisphenol F (BPF) and bisphenol S (BPS), on embryonic development with an in vitro stem cell toxicology system and transcriptomics analyses. Mouse embryonic stem cells (mESCs) were differentiated via embryoid body formation, either globally towards the three primary germ layers and their lineages, or specifically into neuroectoderm/neural progenitor cells. During the differentiation, cells were treated with BPA, BPF, BPS, or DMSO control. Samples were collected at different time points, for qRT-PCR and RNA-seq analyses. BPA, BPF and BPS disrupted many processes, during mESC global and neural differentiations, in very similar manners. In fact, at each time point the three chemicals differentially regulated analogous gene categories, particularly the ones involved in cell-matrix and cell-cell adhesion, signal transduction pathways, and medical conditions such as cardiovascular diseases and cancer. Our findings demonstrate once more then BPA substitutes may not be very safe. They potentially have a very complex developmental toxicity, similarly to BPA, and seem more toxic than BPA itself. In addition, our results reveal that stem cell-based developmental toxicity assays can be very comprehensive.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
LQX2141发布了新的文献求助10
1秒前
睡不醒的喵完成签到,获得积分10
1秒前
juke发布了新的文献求助10
1秒前
2秒前
4秒前
xuening完成签到,获得积分10
5秒前
小疯狗完成签到,获得积分10
6秒前
7秒前
阳光襄发布了新的文献求助10
7秒前
感动书文完成签到,获得积分10
9秒前
情怀应助79采纳,获得10
10秒前
11秒前
Emma完成签到 ,获得积分10
12秒前
12秒前
方半仙完成签到,获得积分10
13秒前
14秒前
小吕小吕发布了新的文献求助10
15秒前
一川烟雨发布了新的文献求助10
15秒前
orixero应助鱼yu采纳,获得10
15秒前
16秒前
CipherSage应助Cheung2121采纳,获得30
18秒前
18秒前
18秒前
连长发布了新的文献求助10
19秒前
正霖发布了新的文献求助50
19秒前
shufessm完成签到,获得积分0
20秒前
怡然的谷蓝完成签到,获得积分10
21秒前
22秒前
22秒前
xia发布了新的文献求助50
23秒前
23秒前
我很懵逼完成签到,获得积分10
23秒前
胥风发布了新的文献求助10
24秒前
诶诶发布了新的文献求助10
24秒前
25秒前
kxy完成签到,获得积分10
25秒前
26秒前
26秒前
27秒前
小吕小吕发布了新的文献求助10
27秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Evolution 3rd edition 1500
保险藏宝图 1000
Lire en communiste 1000
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 700
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 700
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3186040
求助须知:如何正确求助?哪些是违规求助? 2836400
关于积分的说明 8008971
捐赠科研通 2498786
什么是DOI,文献DOI怎么找? 1333889
科研通“疑难数据库(出版商)”最低求助积分说明 636932
邀请新用户注册赠送积分活动 604821