Embryonic stem cell- and transcriptomics-based in vitro analyses reveal that bisphenols A, F and S have similar and very complex potential developmental toxicities

胚胎干细胞 生物 干细胞 转录组 祖细胞 胚状体 细胞生物学 发育毒性 神经干细胞 细胞分化 诱导多能干细胞 遗传学 基因 基因表达 妊娠期 怀孕
作者
Nuoya Yin,Xiaoxing Liang,Shengxian Liang,Siwei Liang,Renjun Yang,Bowen Hu,Zhanwen Cheng,Shuyu Liu,Hengzhi Dong,Sijin Liu,Francesco Faiola
出处
期刊:Ecotoxicology and Environmental Safety [Elsevier]
卷期号:176: 330-338 被引量:46
标识
DOI:10.1016/j.ecoenv.2019.03.115
摘要

Bisphenol A (BPA) is a very versatile industrial chemical. Many reports have associated BPA with several health effects. Some bisphenol alternatives have been introduced to replace BPA in its many applications. However, comprehensive toxicological evaluations for these replacements are still lacking. In this study, we examined the potential effects of BPA, bisphenol F (BPF) and bisphenol S (BPS), on embryonic development with an in vitro stem cell toxicology system and transcriptomics analyses. Mouse embryonic stem cells (mESCs) were differentiated via embryoid body formation, either globally towards the three primary germ layers and their lineages, or specifically into neuroectoderm/neural progenitor cells. During the differentiation, cells were treated with BPA, BPF, BPS, or DMSO control. Samples were collected at different time points, for qRT-PCR and RNA-seq analyses. BPA, BPF and BPS disrupted many processes, during mESC global and neural differentiations, in very similar manners. In fact, at each time point the three chemicals differentially regulated analogous gene categories, particularly the ones involved in cell-matrix and cell-cell adhesion, signal transduction pathways, and medical conditions such as cardiovascular diseases and cancer. Our findings demonstrate once more then BPA substitutes may not be very safe. They potentially have a very complex developmental toxicity, similarly to BPA, and seem more toxic than BPA itself. In addition, our results reveal that stem cell-based developmental toxicity assays can be very comprehensive.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
40873完成签到,获得积分10
1秒前
1秒前
晗月完成签到,获得积分10
2秒前
打打应助QI采纳,获得10
2秒前
4秒前
嗯嗯哈哈发布了新的文献求助10
4秒前
4秒前
大寒给大寒的求助进行了留言
4秒前
wanci应助韦远侵采纳,获得10
4秒前
汉堡包应助小篆采纳,获得10
5秒前
黄小北完成签到,获得积分10
5秒前
QI完成签到,获得积分10
7秒前
所所应助AAA采纳,获得10
7秒前
7秒前
houyunfeng完成签到 ,获得积分10
7秒前
Ava应助阳光的芯采纳,获得10
8秒前
英俊的铭应助wrufhg采纳,获得30
9秒前
王太白完成签到,获得积分10
10秒前
小鱼发布了新的文献求助10
10秒前
打打应助强度采纳,获得10
13秒前
追寻的问安完成签到,获得积分10
14秒前
14秒前
yiyiyiyiyi//完成签到 ,获得积分10
15秒前
15秒前
15秒前
Rosebud完成签到,获得积分10
16秒前
16秒前
orixero应助聪明的远锋采纳,获得10
17秒前
ah完成签到,获得积分10
17秒前
111完成签到,获得积分10
18秒前
18秒前
HHW发布了新的文献求助10
19秒前
qq关闭了qq文献求助
19秒前
诩阽完成签到,获得积分10
19秒前
暮霭沉沉应助包包采纳,获得10
19秒前
uiwh发布了新的文献求助30
20秒前
畅快白亦发布了新的文献求助10
20秒前
like1994发布了新的文献求助10
20秒前
cpli发布了新的文献求助10
21秒前
鱼海寻俞发布了新的文献求助10
22秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Aspects of Babylonian celestial divination: the lunar eclipse tablets of Enūma Anu Enlil 500
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
Dictionary of socialism 350
Mixed-anion Compounds 300
Geochemistry, 2nd Edition 地球化学经典教科书第二版 300
Idoxuridine 260
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3195624
求助须知:如何正确求助?哪些是违规求助? 2844459
关于积分的说明 8050116
捐赠科研通 2509095
什么是DOI,文献DOI怎么找? 1341427
科研通“疑难数据库(出版商)”最低求助积分说明 639124
邀请新用户注册赠送积分活动 608325