Embryonic stem cell- and transcriptomics-based in vitro analyses reveal that bisphenols A, F and S have similar and very complex potential developmental toxicities

胚胎干细胞 生物 干细胞 转录组 祖细胞 胚状体 细胞生物学 发育毒性 神经干细胞 细胞分化 诱导多能干细胞 遗传学 基因 基因表达 妊娠期 怀孕
作者
Nuoya Yin,Xiaoxing Liang,Shengxian Liang,Siwei Liang,Renjun Yang,Bowen Hu,Zhanwen Cheng,Shuyu Liu,Hengzhi Dong,Sijin Liu,Francesco Faiola
出处
期刊:Ecotoxicology and Environmental Safety [Elsevier]
卷期号:176: 330-338 被引量:46
标识
DOI:10.1016/j.ecoenv.2019.03.115
摘要

Bisphenol A (BPA) is a very versatile industrial chemical. Many reports have associated BPA with several health effects. Some bisphenol alternatives have been introduced to replace BPA in its many applications. However, comprehensive toxicological evaluations for these replacements are still lacking. In this study, we examined the potential effects of BPA, bisphenol F (BPF) and bisphenol S (BPS), on embryonic development with an in vitro stem cell toxicology system and transcriptomics analyses. Mouse embryonic stem cells (mESCs) were differentiated via embryoid body formation, either globally towards the three primary germ layers and their lineages, or specifically into neuroectoderm/neural progenitor cells. During the differentiation, cells were treated with BPA, BPF, BPS, or DMSO control. Samples were collected at different time points, for qRT-PCR and RNA-seq analyses. BPA, BPF and BPS disrupted many processes, during mESC global and neural differentiations, in very similar manners. In fact, at each time point the three chemicals differentially regulated analogous gene categories, particularly the ones involved in cell-matrix and cell-cell adhesion, signal transduction pathways, and medical conditions such as cardiovascular diseases and cancer. Our findings demonstrate once more then BPA substitutes may not be very safe. They potentially have a very complex developmental toxicity, similarly to BPA, and seem more toxic than BPA itself. In addition, our results reveal that stem cell-based developmental toxicity assays can be very comprehensive.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
在水一方应助liwj采纳,获得10
1秒前
小墨应助佳贝采纳,获得10
1秒前
1秒前
mirrovo完成签到 ,获得积分10
1秒前
1234567890发布了新的文献求助10
1秒前
2秒前
CodeCraft应助跳跃鱼采纳,获得10
4秒前
善学以致用应助跳跃鱼采纳,获得10
4秒前
天天快乐发布了新的文献求助10
4秒前
5秒前
5秒前
7秒前
fanfan发布了新的文献求助10
7秒前
10秒前
可爱的函函应助Res_M采纳,获得10
11秒前
12秒前
斯文败类应助Aurora采纳,获得10
15秒前
liwj发布了新的文献求助10
16秒前
z1jioyeah完成签到 ,获得积分10
17秒前
香蕉觅云应助luct采纳,获得10
17秒前
17秒前
18秒前
18秒前
HW发布了新的文献求助30
18秒前
很菜的研究生完成签到,获得积分10
19秒前
19秒前
走远了完成签到,获得积分10
20秒前
橙子发布了新的文献求助10
21秒前
ying发布了新的文献求助10
22秒前
CodeCraft应助WANG采纳,获得20
22秒前
23秒前
23秒前
Alibizia发布了新的文献求助10
26秒前
xiaofutongxue发布了新的文献求助10
26秒前
27秒前
luct完成签到,获得积分10
28秒前
北风吹微云完成签到,获得积分10
29秒前
不配.应助飞鸿雪花采纳,获得10
31秒前
luct发布了新的文献求助10
34秒前
劲秉应助炙心采纳,获得30
34秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
ALA生合成不全マウスでの糖代謝異常の分子機構解析 520
安全防范技术与工程 500
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
A real-time energy management strategy based on fuzzy control and ECMS for PHEVs 400
2024 Medicinal Chemistry Reviews 400
Актуализированная стратиграфическая схема триасовых отложений Прикаспийского региона. Объяснительная записка 360
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3191505
求助须知:如何正确求助?哪些是违规求助? 2840825
关于积分的说明 8030408
捐赠科研通 2504207
什么是DOI,文献DOI怎么找? 1337556
科研通“疑难数据库(出版商)”最低求助积分说明 638102
邀请新用户注册赠送积分活动 606622