Embryonic stem cell- and transcriptomics-based in vitro analyses reveal that bisphenols A, F and S have similar and very complex potential developmental toxicities

胚胎干细胞 生物 干细胞 转录组 祖细胞 胚状体 细胞生物学 发育毒性 神经干细胞 细胞分化 诱导多能干细胞 遗传学 基因 基因表达 妊娠期 怀孕
作者
Nuoya Yin,Xiaoxing Liang,Shengxian Liang,Siwei Liang,Renjun Yang,Bowen Hu,Zhanwen Cheng,Shuyu Liu,Hengzhi Dong,Sijin Liu,Francesco Faiola
出处
期刊:Ecotoxicology and Environmental Safety [Elsevier]
卷期号:176: 330-338 被引量:46
标识
DOI:10.1016/j.ecoenv.2019.03.115
摘要

Bisphenol A (BPA) is a very versatile industrial chemical. Many reports have associated BPA with several health effects. Some bisphenol alternatives have been introduced to replace BPA in its many applications. However, comprehensive toxicological evaluations for these replacements are still lacking. In this study, we examined the potential effects of BPA, bisphenol F (BPF) and bisphenol S (BPS), on embryonic development with an in vitro stem cell toxicology system and transcriptomics analyses. Mouse embryonic stem cells (mESCs) were differentiated via embryoid body formation, either globally towards the three primary germ layers and their lineages, or specifically into neuroectoderm/neural progenitor cells. During the differentiation, cells were treated with BPA, BPF, BPS, or DMSO control. Samples were collected at different time points, for qRT-PCR and RNA-seq analyses. BPA, BPF and BPS disrupted many processes, during mESC global and neural differentiations, in very similar manners. In fact, at each time point the three chemicals differentially regulated analogous gene categories, particularly the ones involved in cell-matrix and cell-cell adhesion, signal transduction pathways, and medical conditions such as cardiovascular diseases and cancer. Our findings demonstrate once more then BPA substitutes may not be very safe. They potentially have a very complex developmental toxicity, similarly to BPA, and seem more toxic than BPA itself. In addition, our results reveal that stem cell-based developmental toxicity assays can be very comprehensive.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
SciGPT应助科研通管家采纳,获得10
刚刚
pluto应助科研通管家采纳,获得10
刚刚
pluto应助科研通管家采纳,获得10
刚刚
刚刚
刚刚
CipherSage应助科研通管家采纳,获得10
刚刚
pluto应助科研通管家采纳,获得10
刚刚
1秒前
2秒前
嘟嘟请让一让完成签到,获得积分10
3秒前
爱我嫉妒我完成签到,获得积分20
3秒前
3秒前
whitekitten发布了新的文献求助10
4秒前
VVV完成签到 ,获得积分10
4秒前
4秒前
Justin完成签到,获得积分10
4秒前
gomm完成签到,获得积分10
5秒前
5秒前
纯情的书蝶完成签到,获得积分10
5秒前
6秒前
张本智悦完成签到,获得积分20
7秒前
鑫鑫努力学习完成签到,获得积分10
7秒前
8秒前
8秒前
8秒前
9秒前
高高的雪枫完成签到,获得积分20
10秒前
重要冲发布了新的文献求助10
10秒前
木易心应助YiXianCoA采纳,获得10
11秒前
TOPIC_BOX完成签到,获得积分10
11秒前
Zzz完成签到,获得积分20
11秒前
CometYe发布了新的文献求助10
12秒前
开朗向真完成签到,获得积分10
13秒前
周周发布了新的文献求助30
13秒前
无花果应助huahua采纳,获得10
13秒前
ll完成签到 ,获得积分10
13秒前
nienie发布了新的文献求助10
13秒前
13秒前
13秒前
JamesPei应助zhao采纳,获得10
14秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
ALA生合成不全マウスでの糖代謝異常の分子機構解析 520
Aspects of Babylonian celestial divination: the lunar eclipse tablets of Enūma Anu Enlil 500
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
2024 Medicinal Chemistry Reviews 400
Dictionary of socialism 350
Mixed-anion Compounds 300
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3198098
求助须知:如何正确求助?哪些是违规求助? 2847040
关于积分的说明 8061947
捐赠科研通 2511962
什么是DOI,文献DOI怎么找? 1343804
科研通“疑难数据库(出版商)”最低求助积分说明 639638
邀请新用户注册赠送积分活动 609275