Embryonic stem cell- and transcriptomics-based in vitro analyses reveal that bisphenols A, F and S have similar and very complex potential developmental toxicities

胚胎干细胞 生物 干细胞 转录组 祖细胞 胚状体 细胞生物学 发育毒性 神经干细胞 细胞分化 诱导多能干细胞 遗传学 基因 基因表达 妊娠期 怀孕
作者
Nuoya Yin,Xiaoxing Liang,Shengxian Liang,Siwei Liang,Renjun Yang,Bowen Hu,Zhanwen Cheng,Shuyu Liu,Hengzhi Dong,Sijin Liu,Francesco Faiola
出处
期刊:Ecotoxicology and Environmental Safety [Elsevier]
卷期号:176: 330-338 被引量:46
标识
DOI:10.1016/j.ecoenv.2019.03.115
摘要

Bisphenol A (BPA) is a very versatile industrial chemical. Many reports have associated BPA with several health effects. Some bisphenol alternatives have been introduced to replace BPA in its many applications. However, comprehensive toxicological evaluations for these replacements are still lacking. In this study, we examined the potential effects of BPA, bisphenol F (BPF) and bisphenol S (BPS), on embryonic development with an in vitro stem cell toxicology system and transcriptomics analyses. Mouse embryonic stem cells (mESCs) were differentiated via embryoid body formation, either globally towards the three primary germ layers and their lineages, or specifically into neuroectoderm/neural progenitor cells. During the differentiation, cells were treated with BPA, BPF, BPS, or DMSO control. Samples were collected at different time points, for qRT-PCR and RNA-seq analyses. BPA, BPF and BPS disrupted many processes, during mESC global and neural differentiations, in very similar manners. In fact, at each time point the three chemicals differentially regulated analogous gene categories, particularly the ones involved in cell-matrix and cell-cell adhesion, signal transduction pathways, and medical conditions such as cardiovascular diseases and cancer. Our findings demonstrate once more then BPA substitutes may not be very safe. They potentially have a very complex developmental toxicity, similarly to BPA, and seem more toxic than BPA itself. In addition, our results reveal that stem cell-based developmental toxicity assays can be very comprehensive.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
yangjinru完成签到 ,获得积分10
刚刚
syp发布了新的文献求助10
刚刚
哈哈哈完成签到 ,获得积分20
1秒前
1秒前
fzhou完成签到 ,获得积分10
1秒前
安然发布了新的文献求助10
1秒前
1秒前
完美冷安完成签到,获得积分10
1秒前
苏苏诺诺2023完成签到,获得积分10
1秒前
ZHQ驳回了Jia小哥应助
2秒前
3秒前
actor2006完成签到,获得积分10
3秒前
dream完成签到,获得积分10
4秒前
CipherSage应助默默的雁易采纳,获得10
5秒前
5秒前
5秒前
诗跟离别应助激昂的梦山采纳,获得10
5秒前
taowang14发布了新的文献求助10
6秒前
欧阳万仇发布了新的文献求助10
6秒前
852应助菠萝炒饭采纳,获得150
7秒前
爆米花应助Kindy采纳,获得10
7秒前
7秒前
9秒前
Hwalnut完成签到,获得积分10
9秒前
地球观光客完成签到,获得积分10
10秒前
zhuo发布了新的文献求助10
10秒前
Lee发布了新的文献求助10
11秒前
11秒前
长情的念真完成签到,获得积分20
12秒前
12秒前
12秒前
13秒前
13秒前
完美世界应助刘觅儿采纳,获得30
13秒前
学术废物完成签到,获得积分10
13秒前
xhn完成签到 ,获得积分10
14秒前
ftt完成签到,获得积分20
15秒前
魏魏发布了新的文献求助20
15秒前
evacqy完成签到,获得积分20
15秒前
15秒前
高分求助中
Evolution 3rd edition 1500
保险藏宝图 1000
Lire en communiste 1000
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 700
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 700
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3183902
求助须知:如何正确求助?哪些是违规求助? 2834188
关于积分的说明 7998514
捐赠科研通 2496483
什么是DOI,文献DOI怎么找? 1332259
科研通“疑难数据库(出版商)”最低求助积分说明 636552
邀请新用户注册赠送积分活动 603784