生物
转录组
基因组
遗传学
从头转录组组装
质体
基因
反向重复
系统基因组学
叶绿体DNA
系统发育学
基因表达
叶绿体
克莱德
作者
Hoang Dang Khoa,Joo‐Hwan Kim
标识
DOI:10.1038/s41598-019-43259-7
摘要
Abstract Transcriptome data provide useful information for studying the evolutionary history of angiosperms. Previously, different genomic events (i.e., duplication, deletion, and pseudogenization) were discovered in the plastid genome of Liliales; however, the effects of these events have not addressed because of the lack of transcriptome data. In this study, we completed the plastid genome (cpDNA) and generated transcriptome data of Lilium lancifolium . Consequently, the cpDNA of L . lancifolium is 152,479 bp in length, which consists of one large single copy (81,888 bp), one small single copy (17,607 bp), and two inverted repeat regions (26,544 bp). The comparative genomic analysis of newly sequenced cpDNA and transcriptome data revealed 90 RNA editing sites of which two positions are located in the rRNA coding region of L . lancifolium . A further check on the secondary structure of rRNA showed that RNA editing causes notable structural changes. Most of the RNA editing contents are C-to-U conversions, which result in nonsynonymous substitutions. Among coding regions, ndh genes have the highest number of RNA editing sites. Our study provided the first profiling of plastid transcriptome analyses in Liliales and fundamental information for further studies on post-transcription in this order as well as other petaloid monocotyledonous species.
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