质外体
韧皮部
拟南芥
共济体
植物
拟南芥
染色体易位
木质部
蒸腾流
生物
化学
叶柄(昆虫解剖学)
光合作用
生物化学
细胞壁
蒸腾作用
基因
突变体
膜翅目
作者
Takao Araya,Anne Bohner,Nicolaus Wir�n
出处
期刊:Bio-protocol
[Bio-Protocol]
日期:2015-01-01
卷期号:5 (24)
被引量:11
标识
DOI:10.21769/bioprotoc.1691
摘要
The long-distance translocation of metabolites and mineral elements is crucial for plant growth and reproduction. In most cases, source-to-sink translocation of metabolites and minerals requires their passage through the apoplast, irrespective whether they are transported via the xylem or the phloem. This apoplast-mediated pathway is of particular importance during plant senescence, when photoassimilates as well as organic, inorganic or chelated forms of nutrients are translocated from leaves to fruits or seeds. Recent genetic and physiological studies revealed the involvement of numerous membrane transporters mediating phloem loading of amino acids, sugars, urea or mineral elements. To evaluate the contribution of individual transporters to xylem unloading or phloem loading, the collection of apoplastic fluids and of phloem sap is essential. Here, we describe a method for the extraction of apoplastic fluids and the collection of leaf petiole exudates from Arabidopsis leaves, the latter representing an approximation to the real composition of the phloem sap.
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