纳米载体
赫拉
癌细胞
细胞毒性
化学
运输机
纳米颗粒
癌症研究
癌症
细胞
生物化学
纳米技术
材料科学
生物
体外
基因
遗传学
作者
Lin Li,Xingsheng Di,Mingrui Wu,Zhisu Sun,Lu Zhong,Yongjun Wang,Qiang Fu,Qiming Kan,Jin Sun,Zhonggui He
标识
DOI:10.1016/j.nano.2016.11.012
摘要
Designing active targeting nanocarriers with increased cellular accumulation of chemotherapeutic agents is a promising strategy in cancer therapy. Herein, we report a novel active targeting strategy based on the large amino acid transporter 1 (LAT1) overexpressed in a variety of cancers. Glutamate was conjugated to polyoxyethylene stearate as a targeting ligand to achieve LAT1-targeting PLGA nanoparticles. The targeting efficiency of nanoparticles was investigated in HeLa and MCF-7 cells. Significant increase in cellular uptake and cytotoxicity was observed in LAT1-targeting nanoparticles compared to the unmodified ones. More interestingly, the internalized LAT1 together with targeting nanoparticles could recycle back to the cell membrane within 3 h, guaranteeing sufficient transporters on cell membrane for continuous cellular uptake. The LAT1 targeting nanoparticles exhibited better tumor accumulation and antitumor effects. These results suggested that the overexpressed LAT1 on cancer cells holds a great potential to be a high-efficiency target for the rational design of active-targeting nanosystems.
科研通智能强力驱动
Strongly Powered by AbleSci AI