A comparative study on the free radical scavenging activities of the secondary metabolites of endophytic fungus from Pueraria lobata

We isolated 128 strains of endophytic fungus from the root,stem,leaf and fruit of Pueraria lobata to inspect it free radical scavenging activities by using DPPH-microplate assay and DPPH-TLC assay methods.The results showed that many strains of endophytic fungus have free radical scavenging activities,and the endophytic fungus isolated from different parts of Pueraria lobata and taken from different places have different activities.At the same time,the free radical scavenging activities of the strains are directly in proportion to the extract concentration and reaction time,i.e.the activity increases with the increase of the concentration and its reaction time.The free radical scavenging activity of strain 10y-4 can reaches 92.3% after incubating for 20 min at 2.5 mg·L-1 concentration.The research showed that endophytic fungus taken from Pueraria lobata could be developed and used for natural,high-efficient free radical scavenging agent.